Ontogeny of serotonin-immunoreactive cells in the gut epithelium of the cloudy dogfish, Scyliorhinus torazame, with reference to coexistence of serotonin and neuropeptide Y.
Ontogeny of serotonin-immunoreactive cells in the gut epithelium of the cloudy dogfish, Scyliorhinus torazame, with reference to coexistence of serotonin and neuropeptide Y.
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DOI：
10.1006/gcen.1998.7104
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发表时间：
1998-09
影响因子：
2.7
通讯作者：
A. Chiba
中科院分区：
文献类型：
--
作者：
A. Chiba
用免疫组织化学方法研究了卵生板鳃鱼（Scyliorhinus torazame）肠上皮5-羟色胺（5-HT）免疫反应细胞的个体发生。5-HT-IR细胞首先出现在卵黄肠管（VID）近端和中肠前部（全长30 mm）。在40-mm阶段，细胞数量略有增加，并蔓延到中肠的后部，但在前肠和后肠没有发现标记细胞。到胚胎晚期（74-和80-mm），螺旋肠和胃中5-HT-IR细胞的数量明显增加，而VID和直肠中的5-HT-IR细胞很少。在这些阶段，内卵黄囊中的细胞密度，VID的衍生物，有增加的趋势。这种趋势似乎是一致的，在95毫米阶段的posthatching少年。在幼鱼，125毫米的长度和孵化后1.7个月，细胞进一步增加的数量在螺旋肠，达到其成人的价值。用抗5-HT和抗神经肽Y（NPY）抗血清进行双重免疫染色，结果表明，部分5-HT IR细胞也呈NPY阳性。
The ontogeny of serotonin (5-HT)-immunoreactive (IR) cells in the gut epithelium of an oviparous elasmobranch, Scyliorhinus torazame, was examined immunohistochemically. 5-HT-IR cells first appeared in the proximal part of the vitellointestinal duct (VID) and in the anterior part of the midgut of the embryo (30 mm in total length). At the 40-mm stage, the cells slightly increased in number and spread to the posterior part of the midgut, but no labeled cells were found in the foregut or hindgut. By the late embryonic (74- and 80-mm) stages, 5-HT-IR cells were markedly increased in number in the spiral intestine and stomach, whereas they were few in the VID and rectum. During these stages, the density of the cells in the inner yolk sac, the derivative of the VID, tended to be increased. This tendency seemed to be consistent in the posthatching juveniles at the 95-mm stage. In juveniles, 125 mm in length and 1.7 months after hatching, the cells further increased in number in the spiral intestine, reaching their adult value. Double immunostaining by the use of anti-5-HT and -neuropeptide Y (NPY) antisera demonstrated that some of the 5-HT-IR cells were also positive for NPY.