Stability and in vitro simulated release characteristics of ultrasonically modified soybean lipophilic protein emulsion
Stability and in vitro simulated release characteristics of ultrasonically modified soybean lipophilic protein emulsion
复制标题
超声改性大豆亲脂蛋白乳的稳定性及体外模拟释放特性
DOI：
10.1039/d0fo00238k
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发表时间：
2020-05-01
期刊：
影响因子：
6.1
通讯作者：
Qi, Baokun
中科院分区：
文献类型：
--
作者：
Li, Yang;Wang, Diqiong;Qi, Baokun
天然乳化剂如大豆亲脂蛋白（SLP）显示出作为疏水性生物活性组分如维生素E的递送系统的潜力;然而，SLP的溶解度受到其高脂质含量的限制。研究了不同超声条件对SLP结构和性能的影响。采用改性SLP的乳液，对维生素E的载体性能和体外消化释放性能进行了评价。生化和光谱分析表明，超声波处理主要改变了SLP的二级和三级结构。此外，适当的超声条件显着改善SLP的溶解性和乳化性能，与最高的乳化稳定性和SLP包封率获得使用超声功率为240 W，20 min。体外消化模拟表明，通过超声改性的SLP制备的乳液是一个有效的维生素E的传递系统。特别是，该乳剂保护了维生素E的生物活性，同时显著提高了脂质消化速率和维生素E的生物利用度。这些结果表明，超声改性的SLP可用于制备用于包封维生素E的稳定乳液，这为疏水性生物活性成分的递送提供了新的途径。
Natural emulsifiers such as soybean lipophilic protein (SLP) show potential as delivery systems for hydrophobic bioactive components such as vitamin E; however, the solubility of SLP is limited by its high lipid content. This study evaluated the effects of various ultrasonic conditions on the structure and properties of SLP. Using an emulsion of modified SLP, the carrier properties and in vitro digestion and release properties for vitamin E were evaluated. Biochemical and spectroscopic analyses indicated that the ultrasonic treatment mainly changed the secondary and tertiary structures of SLP. Furthermore, appropriate ultrasonic conditions significantly improved the solubility and emulsifying properties of SLP, with the highest emulsion stability and SLP encapsulation efficiency obtained using an ultrasonic power of 240 W for 20 min. An in vitro digestion simulation revealed that the emulsion prepared by ultrasonic modification of SLP was an effective delivery system for vitamin E. In particular, the emulsion protected the biological activity of vitamin E while significantly increasing the rate of lipid digestion and the bioavailability of vitamin E. These results indicate that the ultrasonically modified SLP can be used to prepare a stable emulsion for encapsulating vitamin E, which provides a new approach for the delivery of hydrophobic bioactive components.