Preparation and characterization of trace elements-multidoped injectable biomimetic materials for minimally invasive treatment of osteoporotic bone trauma.
Preparation and characterization of trace elements-multidoped injectable biomimetic materials for minimally invasive treatment of osteoporotic bone trauma.
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DOI：
10.1002/jbm.a.32936
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发表时间：
2010-12
期刊：
影响因子：
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通讯作者：
Xianyan Yang;Yilai Gan;Xin Gao;Li Zhao;Changyou Gao;Xinli Zhang;Yanbo Feng;K. Ting;Z. Gou
中科院分区：
文献类型：
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作者：
Xianyan Yang;Yilai Gan;Xin Gao;Li Zhao;Changyou Gao;Xinli Zhang;Yanbo Feng;K. Ting;Z. Gou
如何使用合适的植入物或生物材料修复骨质疏松症患者创伤性骨缺损区的功能一直是临床面临的挑战。在本研究中，我们制备了海藻酸盐-壳聚糖/微量元素复合掺杂磷酸八钙-生物玻璃(AT-CS/teOCP-BG)水凝胶复合材料作为可注射生物材料，并对其物理化学性质和生物学性能进行了评价。首先通过湿化学反应将微量的硅、锶和锌掺杂到OCP多孔微球的结构中。AT-CS络合物与TeOCP机械混合，然后被生物玻璃58S颗粒溶解的离子产物中和。这种新型复合材料在湿状态下的配方中，TeOCP-BG的含量为7.8-25.4%，AT-CS的含量低于3.2%。37℃时，复合材料的储能模数与损耗模数之比(G‘/G“)随BG用量的减少而显著增大，表明凝胶的流变性和凝胶稳定性随pH值的增加而增强。卵巢切除(OVXed)大鼠骨髓腔内注射微创组织后，术后8周内X线片上可见新生骨组织明显多于假手术组。通过X线、微电脑断层扫描和组织学分析，观察到去卵巢大鼠的快速骨再生现象。这些结果表明，AT-CS/teOCP-BG系统可以作为一种可注射的生物材料用于骨质疏松症相关(微)创伤的微创治疗。
It has always been a clinical challenge to use the proper implants or biomaterials to restore function to traumatized bone defects in patients with osteoporosis. In this study, we prepared the alginate-chitosan/trace elements-multidoped octacalcium phosphate-bioglass (AT-CS/teOCP-BG) hydrogel composite as an injectable biomaterial and evaluated its physicochemical properties and biological performance. Trace amount of silicon, strontium, and zinc was first doped into the structure of OCP porous microspheres via a wet chemical reaction. The AT-CS complex was mechanically mixed with teOCP and then neutralized by the ionic products of bioglass 58S particles dissolution. Formulations of this novel composite presented in wet state have teOCP-BG contents ranging from 7.8 to 25.4% and AT-CS content of below 3.2%. The composite retained gel in culture medium at 37°C, and the ratio of storage modulus and loss modulus (G'/G″) exhibited a marked increase with decreasing the amount of BG, signifying a pH-dependent enhancement of rheological properties and gel stability. After injection into rat femoral bone marrow cavity with minimal tissue invasion, new bone ingrowth was observed from radiologic images in the ovariectomized (OVXed) rats, which was significantly greater than that found in sham-operated animals within 8 weeks postoperatively. The fast bone regeneration phenomenon was observed in the OVXed rats by radiographic and microcomputerized tomography examination and histological analysis. These findings suggest that the AT-CS/teOCP-BG system might be used as an injectable biomaterial for minimally invasive treatment of osteoporosis-related (micro-)trauma.