Partial purification of pemphigus-related epidermal antigens.
Partial purification of pemphigus-related epidermal antigens.
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天疱疮相关表皮抗原的部分纯化。
DOI：
10.1111/1523-1747.ep12558285
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发表时间：
1978
影响因子：
6.5
通讯作者：
G. Schaumburg‐Lever
中科院分区：
文献类型：
--
作者：
A. Rule;B. Schmidt;M. Kirch;N. Barney;G. Schaumburg‐Lever
通过等电聚焦（pH 5.2峰）或伴刀豆球蛋白A（Con A）亲和层析部分纯化的表皮抗原在管沉淀反应中与Con A反应。在Ouchterlony凝胶中，用兔抗血清（Rab）对pH5.2抗原形成粗皮肤抗原、用α-甲基葡萄糖苷洗脱的Con A亲和抗原和pH5.2峰之间的同一性条带。用A+红细胞吸收Rab或天疱疮抗体（Pab）不会影响Rab与皮肤抗原的补体结合反应，也不会消除Pab与细胞间粘固粉相互作用的能力。pH5.2的表皮抗原在试管沉淀反应中与Pab反应较弱，并且仅较弱地（如果有的话）抑制细胞间粘固剂区域中的Pab反应。高浓度的Con A抑制Pab，过氧化物酶-抗-IgG组织反应，而不发生匡威的抑制。在不抑制Pab的Con A浓度下同时使用Pab和Con A-过氧化物酶反应，会导致细胞间粘固蛋白区域的组织过氧化物酶反应增强。这些初步数据表明，Pab和Con A反应位点位于不同的分子或抗原决定簇的细胞间水泥。他们排除了A血物质参与任何一个部位的可能性。
Epidermal antigens partially purified by either isoelectric focusing (the pH 5.2 peak) or concanavalin A (Con A) affinity chromatography react with Con A in tube precipitation reactions. Bands of identity between crude skin antigens, the Con A affinity antigens eluted with alpha-methyl glucoside and the pH 5.2 peak are formed in Ouchterlony gel with rabbit antisera (Rab) to the pH 5.2 antigen. Absorption of Rab or pemphigus antibodies (Pab) with A+ erythrocytes does not affect complement fixation reactions of Rab with the skin antigen nor abolish the ability of Pab to interact with the intercellular cement. The pH 5.2 epidermal antigens react weakly with Pab in tube precipitation reactions and only weakly, if at all, to inhibit Pab reactions in the region of the intercellular cement. High concentrations of Con A inhibit the Pab, peroxidase-anti-IgG tissue reaction whereas the converse inhibition does not occur. Simultaneous use of both Pab and Con A-perodixase reactions at Con A concentrations which do not inhibit Pab, causes enhanced tissue peroxidase reactions in the region of the intercellular cement. These preliminary data indicate that the Pab and Con A-reacting sites are localized on different molecules or antigenic determinants in the intercellular cement. They exclude the possibility that A-blood substances are involved in either site.