Effects of voluntary exercise on plasma and urinary metabolites and gut microbiota in mice fed with high-fat-diet
Effects of voluntary exercise on plasma and urinary metabolites and gut microbiota in mice fed with high-fat-diet
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DOI：
10.7600/jpfsm.9.205
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发表时间：
2020-09
期刊：
影响因子：
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通讯作者：
C. Watanabe;Nanako Kobiki;Takafumi Aoki;M. J. Kremenik;T. Nagano;H. Yano;Eri Oyanagi
中科院分区：
文献类型：
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作者：
C. Watanabe;Nanako Kobiki;Takafumi Aoki;M. J. Kremenik;T. Nagano;H. Yano;Eri Oyanagi
本研究的目的是检查自愿运动对血浆和尿液代谢产物的影响以及高脂饮食喂养小鼠肠道微生物群的变化。健康雄性C57 BL/6 J小鼠（4周龄，n = 27）在两种条件下喂食正常对照饮食（CD）和高脂饮食（HFD）10周：自愿轮跑（W）和久坐对照条件（C）。使用1H-NMR光谱技术检测采集的血浆和尿液中的代谢物。此外，对收集的粪便进行16 S-rRNA基因下一代测序。HFDW小鼠中的轮跑活动略高于CDW小鼠（p = 0.075）。运动和饮食显著改变了体重、脂肪积累和葡萄糖耐量试验。血浆中Leu、Ile、Ala、Tyr等氨基酸含量随运动时间的延长而增加。饮食影响小鼠血浆和尿液中的代谢物，并显示出显著差异;血浆中为Leu、Ile、Glu、3-HB、乳酸盐和乙酸盐，而尿液中为柠檬酸盐、三甲胺（TMA）、三甲胺-N-氧化物（TMAO）、牛磺酸、马尿酸盐和尿囊素。关于β多样性，未加权UniFrac分析（主坐标分析：PCoA）显示CD和HFD小鼠之间的差异在PC 1下可观察到（22.61%）。尽管两种条件（C与W）之间存在大量重叠，但与C和W条件相比，HFD组位于略微不同的区域。HFD小鼠厚壁菌/拟杆菌的比例显著高于CD小鼠，但不受轮跑的影响，这可能与肥胖有关。我们的研究结果表明，在血浆和尿液中的代谢物的测试可能被证明是一个更可靠的方法，定量代谢物分析的运动依赖性主机的影响，或作为一个独立的改变肠道菌群的主机。
The aim of this study was to examine the effects of voluntary exercise on plasma and urinary metabolites and the changes in gut microbiota in mice fed with a high-fat-diet. Healthy male C57BL/6J mice (four-week-old, n = 27) were fed a normal controlled diet (CD) and a high-fat-diet (HFD) for 10 weeks under two conditions: voluntary wheel running (W) and sedentary controlled condition (C). The metabolites in the collected plasma and urine were detected using 1 H-NMR spectroscopy techniques. Also, 16S-rRNA gene next-generation sequencing was carried out on the collected feces. Wheel running activity in HFDW mice was slightly higher than that in CDW mice (p = 0.075). Exercise and diet significantly altered body weight, fat accumulation, and glucose tolerance tests. In plasma, amino acids such as Leu, Ile, Ala and Tyr, were increased by exercise. Diet influenced the metabolites in both the plasma and urine of mice and showed significant differences; in plasma, Leu, Ile, Glu, 3-HB, lactate and acetate, whereas in urine, citrate, trimethylamine (TMA), trimethylamine-N-oxide (TMAO), taurine, hippurate and allantoin. With regard to beta diversity, unweighted UniFrac analysis (principal coordinate analysis: PCoA) showed the difference between CD and HFD mice could be observed under PC1 (22.61%). Although there was substantial overlap between two conditions (C vs. W), HFD groups were positioned in a slightly different area when compared to the C and W conditions. Firmicutes/Bacteroidetes ratio, which might be associated with obesity, in HFD mice was significantly higher than that in CD mice, but not affected by wheel running. Our results suggest that testing in both plasma and urinary metabolites may prove to be a more reliable approach to quantitative metabolite analysis on the effects of exercise-dependent hosts or as an independent alteration of gut microbiota on the hosts.