The extraction and characterization of porous HA/β-TCP biphasic calcium phosphate from sole fish bones at different temperatures
The extraction and characterization of porous HA/β-TCP biphasic calcium phosphate from sole fish bones at different temperatures
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DOI：
10.1088/2053-1591/ab5a8f
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发表时间：
2019-12
影响因子：
2.3
通讯作者：
Haiwen Chen;Wenxue Dou;Qingfeng Zhu;D. Jiang;Jinfeng Xia;Xingang Wang;Weizhong Tang;Shaohai Wang
中科院分区：
文献类型：
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作者：
Haiwen Chen;Wenxue Dou;Qingfeng Zhu;D. Jiang;Jinfeng Xia;Xingang Wang;Weizhong Tang;Shaohai Wang
如今，BCP 被公认为骨重建手术中骨替代品的黄金标准。研究表明，从海洋鱼骨中提取的BCP具有制备方法简单、煅烧温度低、天然微孔结构、β-TCP含量高等优点。因此，本研究的目的是从海洋鱼骨（鳎鱼）中提取BCP，并研究其成分和微观结构特征。我们将鳎鱼骨在700℃至1100℃下进行煅烧，然后使用X射线衍射（XRD）、傅里叶变换红外光谱（FTIR）、电感耦合等离子体光学原子发射光谱（ICP-OES）、扫描电子显微镜（SEM）和压汞孔隙率计（MIP）对煅烧材料的成分和微观结构进行表征。结果表明，BCP材料在800℃以上观察到，具有丰富的大孔和微孔，晶粒尺寸为纳米级，孔隙率较高。 700℃~1000℃时,β-TCP相对含量随温度升高而增加,1000℃时β-TCP相对含量最高。随着温度的升高，晶粒尺寸和微孔尺寸逐渐增大，比表面积逐渐减小，而大孔尺寸没有明显变化。孔隙率在800℃以下无明显变化，在800℃～900℃时显着增加，在900℃以上逐渐下降。这些结果表明，鳎鱼骨可以在较低的温度下煅烧，提取出具有大量β-TCP和良好微孔结构的BCP材料。鳎鱼骨未来有可能作为生物陶瓷骨替代支架应用于临床。
Nowadays, BCPs are recognized as the gold standard of bone substitutes in bone reconstructive surgery. Studies have shown that BCP derived from some marine fishbones have the advantages of simple preparation method, low calcination temperature, natural micro-pore structure and high β-TCP content. Therefore, the purpose of this study is to extract BCP from a marine fish bone (sole fish) and to research the characteristics of the composition and microstructure. We calcined the sole fish bones at 700 °C to 1100 °C, and then x-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), inductively coupled plasma optical atom emissions spectroscopy (ICP-OES), scanning electron microscopy (SEM) and mercury intrusion porosimeter (MIP) were used to characterize the composition and microstructure of calcined material. The results showed that BCP material was observed above 800 °C, with abundant macropores and micropores, crystalline grain size at nanometer level and high porosity. The relative content of β-TCP was increased with the rise of temperature at 700 °C to 1000 °C, to the highest at 1000 °C. With the rise of temperature, the crystalline grain size and micropore size were gradually increased, and the specific surface area was gradually decreased, while there was no significant change in the macropore size. The porosity showed no significant change below 800 °C, significantly increased at 800 °C to 900 °C and gradually decreased above 900 °C. These results suggest that the sole fish bone can be calcined at a relatively lower temperature to extract BCP material with a large amount of β-TCP and a good micro-pore structure. Sole fish bone will be potentially used as a bioceramic bone alternative scaffold in the clinical practice in the future.