Enterobacteria Phage Ac3's Genome Annotation and Host Range Analysis Against the ECOR Reference Library.
Enterobacteria Phage Ac3's Genome Annotation and Host Range Analysis Against the ECOR Reference Library.
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肠杆菌噬菌体 Ac3 的基因组注释和针对 ECOR 参考库的宿主范围分析。
DOI：
10.1089/phage.2022.0008
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发表时间：
2022
期刊：
影响因子：
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通讯作者：
Nugen,SamR
中科院分区：
文献类型：
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作者：
Farquharson,EmmaL;Nugen,SamR
噬菌体分离株的宿主范围分析和基因组测序/注释允许更有效地开发用于医学，农业和环境中的应用的工具，并扩展我们对噬菌体生物学的理解。在这里，我们提出了完整的序列噬菌体Ac 3的组装和注释的基因组（登录OK 040907）。Ac 3最初简称为“3”，以前曾被描述为T4样噬菌体，属于尾噬菌体目中的肌尾病毒科。使用斑点试验和全板空斑试验的组合，Ac 3的容许和吸附宿主范围根据ECOR参考文库进行评估;一组72株大肠杆菌分离株代表了E.杆菌斑点试验表明，Ac 3可以吸附到43的72株（59.7%），而空斑试验表明，Ac 3的能力，完成复制的27 72株（37.5%）。通过叠加斑点试验和空斑试验结果，45种非允许ECOR菌株中的16种（35.5%）被强调能够支持Ac 3的吸附和尾部收缩，但不能支持其复制。Ac 3的进一步表征仍然需要，然而，这里提出的研究为未来的研究提供了一个坚实的起点。
Host range analyses and genome sequencing/annotation of bacteriophage isolates allow more effective development of tools for applications in medicine, agriculture, and the environment and expand our understanding of phage biology. Here we present the complete sequence of phage Ac3's assembled and annotated genome (accession OK040907). Originally referred to simply as “3,” Ac3 has previously been described as a T4-like bacteriophage belonging to theMyoviridaefamily in theCaudoviralesorder of tailed bacteriophages. Using a combination of spot tests and full plate plaque assays, Ac3's permissive and adsorptive host range were evaluated against the ECOR Reference Library; a panel of 72Escherichia coliisolates meant to represent the diversity ofE. coli. Spot assays revealed that Ac3 could adsorb to 43 of the 72 strains (59.7%), whereas plaque assays demonstrated Ac3's ability to complete replication within 27 of the 72 strains (37.5%). By overlaying spot test and plaque assay results, 16 of the 45 nonpermissive ECOR strains (35.5%) were highlighted as being able to support Ac3's adsorption and tail contraction, but not its replication. Further characterization of Ac3 is still needed, however, the study presented here provides a solid starting point for future research.