Cloning and expression analyses of a unique IgT in ayu Plecoglossus altivelis
Cloning and expression analyses of a unique IgT in ayu Plecoglossus altivelis
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DOI：
10.1007/s12562-014-0820-0
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发表时间：
2015-05
影响因子：
1.9
通讯作者：
G. Kato;T. Takano;T. Sakai;T. Matsuyama;N. Sano;C. Nakayasu
中科院分区：
文献类型：
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作者：
G. Kato;T. Takano;T. Sakai;T. Matsuyama;N. Sano;C. Nakayasu
克隆了一种独特的免疫球蛋白重链（Ig） T，并利用定量RT-PCR和原位杂交技术将其在体内的表达与IgM和IgD的表达进行了比较。其他硬骨鱼IgT中的三个免疫球蛋白结构域，分别为CH1、CH2和CH4，在重链中保守。脾脏、头肾、干肾和鳃组织中IgT mRNA水平高于肠、肝和皮肤组织。IgM转录本是所有组织中最丰富的转录本。在原位杂交研究中，IgT和IgM mrna阳性细胞分散在健康鱼的干肾中，而IgD mrna阳性细胞未检测到。注射鳗弧菌后，鳃部和干肾IgT和IgM mRNA水平显著升高，而IgD mRNA水平保持不变。这些结果表明，这种独特的IgT在少数细胞中表达，并在浸入式疫苗接种诱导的免疫应答中发挥作用。
A unique heavy chain of immunoglobulin (Ig) T was cloned from ayuPlecoglossus altivelis, and its in vivo expression was compared with IgM and IgD expression using quantitative RT-PCR and in situ hybridization. Three immunoglobulin domains, corresponding to CH1, CH2, and CH4, in IgT of other teleost fish species were conserved in the heavy chain. IgT mRNA levels were higher in the spleen, head kidney, trunk kidney, and gill tissue than in the intestine, liver, and skin tissue. IgM transcripts were the most abundant transcripts in all tissues tested. In the in situ hybridization studies, IgT and IgM mRNA-positive cells were scattered across the trunk kidney of healthy fish, while IgD mRNA-positive cells were not detected. mRNA levels of IgT and IgM were significantly upregulated in the gills and trunk kidney after vaccination withVibrio anguillarumbacterin, but the IgD mRNA level remained unchanged. These results suggest that this unique IgT is expressed in a small number of cells and that it plays a role in immune responses induced by immersion vaccination.