VARIATION OF POLAR LIPID COMPOSITION OF BACILLUS-SUBTILIS (MARBURG) WITH DIFFERENT GROWTH CONDITIONS
VARIATION OF POLAR LIPID COMPOSITION OF BACILLUS-SUBTILIS (MARBURG) WITH DIFFERENT GROWTH CONDITIONS
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DOI：
10.1016/0014-5793(72)80398-3
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发表时间：
1972-01-01
期刊：
影响因子：
3.5
通讯作者：
ABDOLRAHIMZADEH, H
中科院分区：
文献类型：
--
作者：
MINNIKIN, DE;BADDILEY, J;ABDOLRAHIMZADEH, H
先前对枯草芽孢杆菌（马尔堡）极性脂质的研究表明，除了糖脂之外，还可能存在至少五种磷脂[11。该报告表明，通过控制生长条件，可以改变该生物体的脂质成分并大大简化。在分批培养中，在明显磷酸盐饥饿的条件下，观察到磷脂和无磷酸盐极性脂质的密切相互关系，后者随着生长的进展按比例增加。某些恒化培养物（镁限制）被发现仅含有一种酸性磷脂、磷脂酰甘油（PC）和一种中性极性脂质、二葡萄糖二甘油酯（DG）。在限制磷酸盐的培养物中，磷脂的比例减少，并且观察到无磷酸盐的极性脂质的比例增加；这些脂质之一是 DG，另一种是酸性肽脂。血管。用氯仿-甲醇（2：1，v/v）从冻干细胞中提取脂质，并通过二维薄层色谱法进行研究[4]（详细图2）。通过使用特定的喷雾试剂[2]对脂质进行定性鉴定，并且密度测定法[6]给出了脂质相对比例的近似测量。分批培养的枯草芽孢杆菌（马尔堡）的脂质包括先前研究[I]中报道的磷脂，即二磷脂酰甘油（DPG）、磷脂酰乙醇胺（PE）、PG及其微量赖氨酸酯。在所有培养物中都鉴定出了 DG，并且在一些提取物中还发现了额外的无磷脂质 (X)（见图 1）。初步调查
A previous study of the polar lipids of Bacillus subtilis (Marburg) has shown that at least five phospholipids in addition to glycolipid may be present [11. The report shows that by manipulation of growth conditions the lipid composition of this organism can be varied and greatly simplified. In batch cultures, under conditions of apparent phosphate starvation, a close interrelation of phospholipids and phosphate-free polar lipids is observed, the latter increasing in proportion as growth progresses. Certain chemostat cultures (magnesium limitation) are found to contain only one acidic phospholipid, phosphatidylglycerol(PC), and one neutral polar lipid, diglucosyldiglyceride (DG). In phosphate-limited cultures the proportions of phospholipids are reduced and phosphate-free polar lipids are observed in increased proportions; one of these lipids is DG, the other is an acidic peptidolipid. vessel. Lipids were extracted from lyophilized cells with chloroform-methanol(2: 1, v/v) and investigated by two-dimensional thin-layer chromatography [4](fig. 2 for details). Qualitative identifications of lipids was made by use of specific spray reagents [2], and densitometry [6] gave an approximate measure of the relative proportions of the lipids. The lipids of B. subtilis (Marburg) grown in batch culture included the phospholipids reported in previous studies [I] ie diphosphatidylglycerol(DPG), phosphatidylethanolamine(PE), PG and traces of its lysine ester. DG was identified in all cultures, and in some extracts an additional phosphorous-free lipid (X) was found (see fig. 1). Preliminary investigations