16S rRNA gene sequencing reveals altered composition of gut microbiota in individuals with kidney stones
16S rRNA gene sequencing reveals altered composition of gut microbiota in individuals with kidney stones
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16S rRNA 基因测序揭示肾结石患者肠道微生物群组成的改变
DOI：
10.1007/s00240-018-1037-y
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发表时间：
2018-11-01
期刊：
影响因子：
3.1
通讯作者：
Mo, Zengnan
中科院分区：
文献类型：
--
作者：
Tang, Ruiqiang;Jiang, Yonghua;Mo, Zengnan
肾结石是一种常见的泌尿系统疾病，发病率高，复发率高。表征肾结石患者的肠道微生物群特征可能为该疾病提供有价值的见解和潜在的生物标志物。因此，我们利用16S核糖体RNA （rRNA）基因测序技术探讨了肠道微生物组与肾结石的关系。招募了13名多发性肾结石患者和13名匹配的健康对照者。肾结石患者中观察到的物种数量呈减少趋势，但未达到统计学意义（p= 0.086）。通过β多样性分析发现，肾结石患者与健康对照者之间存在明显的差异。有20个属的相对丰度在肾结石患者和健康对照组之间有显著差异（均p< 0.05）。在20个属中，Phascolarctobacterium、Parasutterella、Ruminiclostridium_5、丹毒杆菌（Erysipelatoclostridium）、fusicatenibacter和dorea与血液中钾、钠、钙、氯等微量元素的浓度相关。通过线性判别分析效应大小（LEfSe）确定肾结石患者的特征微生物组。这些发现可能为肾结石提供新的、无创的潜在诊断生物标志物，并从维持肠道微生态平衡的角度对肾结石的预防和治疗做出贡献。
Nephrolithiasis is a common urological disease with high prevalence and recurrence rates. Characterizing gut microbiome profiles of nephrolithiasis patients may provide valuable insights and potential biomarkers for the disease. Therefore, we explored the relation between gut microbiome and nephrolithiasis using 16S ribosomal RNA (rRNA) gene sequencing. 13 patients with multiple kidney stones and 13 matched healthy controls were recruited. A decreasing trend in number of observed species in nephrolithiasis patients was detected, although statistical significance was not reached (p= 0.086). The inter-group variability in community structure by beta diversity analysis showed a clear separation between nephrolithiasis patients and healthy controls. Twenty genera differentiated significantly in relative abundance between nephrolithiasis patients and healthy controls (allp< 0.05). Among the 20 genera,Phascolarctobacterium, Parasutterella, Ruminiclostridium_5, Erysipelatoclostridium, FusicatenibacterandDoreawere correlated with the concentration of the trace elements in blood, including potassium, sodium, calcium and chlorinum. Characteristic microbiome in nephrolithiasis patients was also identified by linear discriminant analysis effect size (LEfSe). These findings may provide novel and non-invasive potential diagnostic biomarkers for nephrolithiasis, and contribute to prevention and treatment of nephrolithiasis from the perspective of maintaining micro-ecological equilibrium in gut.