Effects of Ortho-dihydroxyisoflavone Derivatives from Korean Fermented Soybean Paste on Melanogenesis in B16 Melanoma Cells and Human Skin Equivalents
Effects of Ortho-dihydroxyisoflavone Derivatives from Korean Fermented Soybean Paste on Melanogenesis in B16 Melanoma Cells and Human Skin Equivalents
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DOI：
10.1002/ptr.3682
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发表时间：
2012-08-01
影响因子：
7.2
通讯作者：
Na, Yong-Joo
中科院分区：
文献类型：
--
作者：
Goh, Myeong-Jin;Park, Jun-Seong;Na, Yong-Joo
在这项研究中，我们研究了8 '-羟基黄豆苷元和3'-羟基黄豆苷元，两个邻二羟基黄豆苷元衍生物从韩国发酵大豆酱，对黑素生成的抑制作用和可能的作用机制B16小鼠黑色素瘤细胞。在B16黑色素瘤细胞中，两种羟基大豆苷元减少了曲酸（一种经证实的增白剂）治疗后黑色素的合成。此外，当用羟基大豆苷元处理体外人皮肤等同物时，相对于曲酸对照，黑素生成的水平显著降低。RT-PCR结果表明，脱色是由于转录抑制的几个黑素生成基因，包括小眼相关转录因子（MITF），由羟基黄豆苷元。免疫印迹结果证实，MITF表达的减少随后降低酪氨酸酶和酪氨酸酶相关蛋白1和2的表达。累积起来，这些结果表明，羟基大豆苷元将是黑色素合成的有效衰减剂以及人类皮肤色素沉着过度的有效抑制剂。版权所有（c）2011约翰威利父子有限公司
In this study we investigated the inhibitory effects and possible mechanisms of action of 8'-hydroxydaidzein and 3'-hydroxydaidzein, two ortho-dihydroxyisoflavone derivatives from Korean fermented soybean paste, on melanogenesis in B16 murine melanoma cells. The two hydroxydaidzeins reduced melanin synthesis comparably to treatment with kojic acid, a proven whitening agent, in B16 melanoma cells. Furthermore, when in vitro human skin equivalents were treated with the hydroxydaidzeins, the levels of melanogenesis were significantly reduced relative to a kojic acid control. The RT-PCR results demonstrated that depigmentation was due to transcriptional repression of several melanogenesis genes, including microphthalmia-associated transcription factor (MITF), by the hydroxydaidzeins. The immunoblotting results confirmed that diminution of MITF expression subsequently decreased expression of tyrosinase, and tyrosinase-related proteins 1 and 2. Cumulatively, these results suggest that hydroxydaidzeins would be potent attenuators of melanin synthesis as well as effective inhibitors of hyperpigmentation in human skin. Copyright (c) 2011 John Wiley & Sons, Ltd.