Programmable 3D Hexagonal Geometry of DNA Tensegrity Triangles
Programmable 3D Hexagonal Geometry of DNA Tensegrity Triangles
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DNA 张拉整体三角形的可编程 3D 六边形几何形状
DOI：
10.1002/anie.202213451
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发表时间：
2023
期刊：
影响因子：
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通讯作者：
Sha, Ruojie
中科院分区：
文献类型：
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作者：
Lu, Brandon;Woloszyn, Karol;Ohayon, Yoel P.;Yang, Bena;Zhang, Cuizheng;Mao, Chengde;Seeman, Nadrian C.;Vecchioni, Simon;Sha, Ruojie
DNA中的非正则相互作用在DNA纳米技术中仍处于探索阶段。最近，发现了许多具有非规范基序的结构，特别是通过非规范粘性末端相互作用形成的典型菱形DNA张紧整体三角形的六边形排列。在这里，我们发现了一系列的机制来编程一个六边形的安排使用：粘性端序列;三角形边缘的扭转应力;和结晶条件。我们展示了沃森-克里克和非典型粘性末端之间的相互作用，其中两者之间的比例决定了晶体形式的分离或组合成复合晶体。最后，我们开发了一种方法，用于重新配置形成的晶体从菱形到六边形的长程几何形状，反之亦然。这些数据证明了对非典型基序及其拓扑自组装的精细控制。这将极大地提高合理设计的DNA构建体的可编程性、功能性和多功能性。
Non‐canonical interactions in DNA remain under‐explored in DNA nanotechnology. Recently, many structures with non‐canonical motifs have been discovered, notably a hexagonal arrangement of typically rhombohedral DNA tensegrity triangles that forms through non‐canonical sticky end interactions. Here, we find a series of mechanisms to program a hexagonal arrangement using: the sticky end sequence; triangle edge torsional stress; and crystallization condition. We showcase cross‐talking between Watson–Crick and non‐canonical sticky ends in which the ratio between the two dictates segregation by crystal forms or combination into composite crystals. Finally, we develop a method for reconfiguring the long‐range geometry of formed crystals from rhombohedral to hexagonal and vice versa. These data demonstrate fine control over non‐canonical motifs and their topological self‐assembly. This will vastly increase the programmability, functionality, and versatility of rationally designed DNA constructs.