Genotyping of HLA-I and HLA-II alleles in Chinese patients with paraneoplastic pemphigus
Genotyping of HLA-I and HLA-II alleles in Chinese patients with paraneoplastic pemphigus
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DOI：
10.1111/j.1365-2133.2007.08361.x
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发表时间：
2008-03-01
影响因子：
10.3
通讯作者：
Zhu, X-J.
中科院分区：
文献类型：
--
作者：
Liu, Q.;Bu, D-F.;Zhu, X-J.
背景 I类和II类HLA基因被认为在寻常型天疱疮和落叶型天疱疮等大疱性皮肤病的免疫发病机制中发挥作用，但我们对中国患者副肿瘤性天疱疮（PNP）的遗传背景知之甚少。目的通过基因分型鉴定中国PNP患者的I类和II类HLA等位基因，并找出HLA等位基因与疾病之间可能的关联方法 19名中国PNP患者纳入本研究。采用聚合酶链式反应和颜色编码序列特异性寡核苷酸探针方法对 HLA-A、B、C、DRB1 和 DQB1 等位基因进行分型。结果 相比之下，中国汉族 PNP 患者中 HLA-B*4002/B*4004、B*51、B*52、Cw*14、DQB1*0301、DRB1*08 和 DRB1*11 的频率相对较高。与正常控制。经过多重比较校正后，Cw*14在这些患者中仍具有统计学意义，而其他等位基因在这些患者中无显着性。结论不同种族和地区的患者易患PNP的遗传背景可能不同。 HLA-Cw*14可能是中国患者PNP的易感等位基因，与法国PNP患者的易感等位基因以及寻常型天疱疮和落叶型天疱疮的易感等位基因不同。
Background Class I and class II HLA genes are thought to play a role in the immunopathogenesis of bullous dermatoses such as pemphigus vulgaris and pemphigus foliaceus, but we know little about the genetic background of paraneoplastic pemphigus (PNP) in Chinese patients.Objectives To identify class I and class II HLA alleles by genotyping in Chinese patients with PNP, and to find out the possible association between HLA alleles and disease susceptibility.Methods Nineteen Chinese patients with PNP were enrolled in this study. HLA-A, B, C, DRB1 and DQB1 alleles were typed by polymerase chain reaction and a colour-coded sequence-specific oligonucleotide probes method.Results The frequencies of HLA-B*4002/B*4004, B*51, B*52, Cw*14, DQB1*0301, DRB1*08 and DRB1*11 were relatively prevalent in Chinese Han patients with PNP in comparison with normal controls. After correction for multiple comparisons, Cw*14 remained statistically significant, and the other alleles were unremarkable in these patients.Conclusions The genetic background predisposing to PNP may be different in patients from various races and areas. HLA-Cw*14 may be the predisposing allele to PNP in Chinese patients, which is different from the predisposing allele in French patients with PNP and the alleles predisposing to pemphigus vulgaris and pemphigus foliaceus.