Lineage- and stage-specific expressed CYCD7;1 coordinates the single symmetric division that creates stomatal guard cells
Lineage- and stage-specific expressed CYCD7;1 coordinates the single symmetric division that creates stomatal guard cells
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DOI：
10.1242/dev.160671
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发表时间：
2017-10
期刊：
影响因子：
4.6
通讯作者：
Annika K. Weimer;Juliana L. Matos;Nidhi Sharma;Farah Patell;James A. H. Murray;W. Dewitte;D. Bergmann
中科院分区：
文献类型：
--
作者：
Annika K. Weimer;Juliana L. Matos;Nidhi Sharma;Farah Patell;James A. H. Murray;W. Dewitte;D. Bergmann
摘要植物的细胞由刚性的细胞壁固定在适当的位置，通常利用空间和时间上不同的细胞分裂程序来组织和维持器官。这就引出了一个问题，即发育调节因子如何与细胞周期机制相互作用，将细胞分裂事件与特定的发育轨迹联系起来。在拟南芥叶片中，气孔（介导植物-大气气体交换的两细胞表皮阀）的发育依赖于一系列定向干细胞样不对称分裂，随后是单个对称分裂。气孔谱系嵌入在一个组织中，其中其他细胞从增殖到有丝分裂后分化的过渡更早，需要气孔谱系特异性因子来延长分裂能力。我们发现，D型细胞周期蛋白，CYCD 7;1，是专门表达的对称保卫细胞形成分裂之前，它是限制这种分裂。此外，我们发现，CYCD 7;1是能够促进分裂在多种情况下，可能通过RBR 1依赖性促进G1/S转换，但CYCD 7;1是在转录水平上的细胞类型特异性转录因子，限制其表达到适当的发展窗口。突出显示的文章：气孔转录因子定义GMC特异性表达的D型细胞周期蛋白CYCD 7;1，促进G1/S过渡与RBR 1的伙伴关系，使细胞周期控制和发展之间的直接联系在拟南芥叶片的命运决定。
ABSTRACT Plants, with cells fixed in place by rigid walls, often utilize spatial and temporally distinct cell division programs to organize and maintain organs. This leads to the question of how developmental regulators interact with the cell cycle machinery to link cell division events with particular developmental trajectories. In Arabidopsis leaves, the development of stomata, two-celled epidermal valves that mediate plant-atmosphere gas exchange, relies on a series of oriented stem cell-like asymmetric divisions followed by a single symmetric division. The stomatal lineage is embedded in a tissue in which other cells transition from proliferation to postmitotic differentiation earlier, necessitating stomatal lineage-specific factors to prolong competence to divide. We show that the D-type cyclin, CYCD7;1, is specifically expressed just prior to the symmetric guard cell-forming division, and that it is limiting for this division. Further, we find that CYCD7;1 is capable of promoting divisions in multiple contexts, likely through RBR1-dependent promotion of the G1/S transition, but that CYCD7;1 is regulated at the transcriptional level by cell type-specific transcription factors that confine its expression to the appropriate developmental window. Highlighted Article: Stomatal transcription factors define GMC-specific expression of the D-type cyclin CYCD7;1, which promotes the G1/S transition in partnership with RBR1, enabling a direct link between cell cycle control and developmental fate decisions in Arabidopsis leaves.