Volume dependent polymorphonuclear leukocytes fractions isolated by counterflow centrifugal elutriation: PMN volume does not correlate with PMN age.
Volume dependent polymorphonuclear leukocytes fractions isolated by counterflow centrifugal elutriation: PMN volume does not correlate with PMN age.
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通过逆流离心淘析分离出体积依赖性多形核白细胞组分：PMN 体积与 PMN 年龄无关。
DOI：
10.1002/jlb.41.6.518
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发表时间：
1987
影响因子：
5.5
通讯作者：
Dodson,RW
中科院分区：
文献类型：
--
作者：
Berkow,RL;Dodson,RW
我们以前报道过，使用逆流离心洗脱（CCE），人类外周血多形核白细胞可以分离成至少六个体积依赖的部分。更大的体积与趋化肽F-met-leu-phe （FMLP）或肿瘤促进剂phorbol肉豆酸酯醋酸酯（PMA）释放的超氧化物增加有关。为了帮助解释这些发现，我们检查了体积依赖性PMN分数（VDPF）中与PMN年龄相关的PMN功能，即碱性磷酸酶活性、磷酸化颗粒的吞噬、对塑料的粘附以及向FMLP和酶酶酶激活血清的定向运动。PMN碱性磷酸酶活性在最小的PMN中较低，在中等大小的PMN中升高，在功能最大的PMN中又下降。PMN吞噬活性也有类似的关系。VDPF对塑料的粘附性和趋化性无显著差异。此外，VDPF在受体数量或配体FMLP或二丁酸磷的结合亲和力方面没有差异。然而，与最小的VDPF相比，最大的NADPH依赖性氧化酶的总细胞活性高出200%。由于“年龄”相关的PMN功能与PMN体积之间缺乏一致的相关性，因此PMN体积不太可能代表PMN年龄的表现。在较大的VDPF中，氧化酶活性的增加很可能是先前提到的更快的氧化爆发的原因。
We have previously reported that human peripheral blood polymorphonuclear leukocytes can be separated into at least six volume dependent fractions using counterflow centrifugal elutriation (CCE). Larger volume was shown to correlate with increased superoxide release with either the chemotactic peptide F-met-leu-phe (FMLP) or the tumor promotor phorbol myristate acetate (PMA). To help explain these findings we have examined volume dependent PMN fractions (VDPF) for PMN functions felt to be correlated to PMN age, ie, alkaline phosphatase activity, phagocytosis of opsonized particles, adherence to plastic, and directed movement to FMLP and zymosan activated serum. PMN alkaline phosphatase activity was found to be low in the smallest PMNs, increasing in the PMNs of intermediate size, and then decreasing again in the largest PMN functions. A similar relationship was noted for PMN phagocytic activity. No significant difference among VDPF in adherence to plastic or Chemotaxis was found. Furthermore, no difference among VDPF was seen for receptor number or binding affinity of the ligands FMLP or phorbol dibutyrate. Total cellular activity of the NADPH dependent oxidase was, however, 200% greater in the largest compared to the smallest VDPF. Because of the lack of consistent correlation between “age” related PMN function and PMN volume, it is unlikely that PMN volume represents a manifestation of PMN age. It is likely that the increased oxidase activity seen among larger VDPF accounts for the more rapid oxidative burst previously noted.