Site-specific recombinases for manipulation of the mouse genome.
Site-specific recombinases for manipulation of the mouse genome.
复制标题
DOI：
10.1007/978-1-60327-019-9_16
复制
发表时间：
2009-01-01
期刊：
影响因子：
--
通讯作者：
Warot, Xavier
中科院分区：
文献类型：
--
作者：
Birling, Marie-Christine;Gofflot, Francoise;Warot, Xavier
位点特异性重组系统是所有对操纵小鼠基因组感兴趣的科学家广泛使用的工具。在本章中，我们重点介绍了利用位点特异性重组酶（SSR）来阐明小鼠基因的功能。第一部分综述了最常用的SSR标记Cre和Flp，以及新近发展起来的Dre和PhiC31，并介绍了诱导型SSR标记系统。由于经验表明，这些系统并不像预期的那样简单，因此特别注意与其生产和应用有关的事实和人工制品，以研究小鼠基因组。在本章的下一部分中，我们将介绍SSR的新应用，包括FLEX和RMCE策略，这些应用使得小鼠基因组的工程设计越来越精确。我们总结并建议了一个工作流程，当使用SSR创建您感兴趣的小鼠模型时可以遵循该流程。总之，这些策略和程序为各种各样的研究提供了基础，这些研究最终将导致在小鼠细胞水平上分析基因的功能。
Site-specific recombination systems are widespread and popular tools for all scientists interested in manipulating the mouse genome. In this chapter, we focus on the use of site-specific recombinases (SSR) to unravel the function of genes of the mouse. In the first part, we review the most commonly used SSR, Cre and Flp, as well as the newly developed systems such as Dre and PhiC31, and we present the inducible SSR systems. As experience has shown that these systems are not as straightforward as expected, particular attention is paid to facts and artefacts associated with their production and applications to study the mouse genome. In the next part of this chapter, we illustrate new applications of SSRs that allow engineering of the mouse genome with more and more precision, including the FLEX and the RMCE strategies. We conclude and suggest a workflow procedure that can be followed when using SSR to create your mouse model of interest. Together, these strategies and procedures provide the basis for a wide variety of studies that will ultimately lead to the analysis of the function of a gene at the cellular level in the mouse.