PARTITION OF PROTEINS IN LIQUID POLYMER-POLYMER 2-PHASE SYSTEMS
PARTITION OF PROTEINS IN LIQUID POLYMER-POLYMER 2-PHASE SYSTEMS
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DOI：
10.1038/182709a0
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发表时间：
1958-01-01
期刊：
影响因子：
64.8
通讯作者：
ALBERTSSON, PA
中科院分区：
文献类型：
--
作者：
ALBERTSSON, PA
液体两相系统中蛋白质的 HE 行为越来越受到人们的关注，因为分配色谱和逆流萃取已被证明是蛋白质混合物分级分离的有用工具，从而补充了吸附色谱和区带电泳**。适合蛋白质的两相系统很难构建，主要是因为（a）大多数蛋白质很容易被通常用于制备相系统的有机溶剂变性或沉淀，以及（b）蛋白质是大分子，因此通常倾向于仅集中在一相中，即获得非常高或非常低的分配系数。有时，当两种蛋白质集中在不同的相中时，这当然是一个优势，因为它会导致较大的分离因子。然而，如果两种蛋白质进入同一相，则很难应用分配色谱法或逆流萃取来进行分离。为了克服这些困难，已经使用了相系统，该系统是通过将水、盐和可溶于水的有机溶剂（如醇、乙二醇醚等*”混合而制成的），其比例使得组成接近辫点。此类系统溶解许多蛋白质并给出有限的分配系数。然而，在辫点附近，分配系数对蛋白质组成的微小变化极其敏感。因此，必须非常小心地控制组成和温度。该通讯描述了当蛋白质分布在由水和可溶于水的两种高聚合物组成的两相系统之间时获得的一些结果，该系统也包含对细胞颗粒的分级的参考。
HE behaviour of proteins in liquid two-phase systems has been of increasing interest because partition chromatography and counter-current ex-traction have been shown to be useful tools for the fractionation of protein mixtures, thus complementing adsorption chromatography and Zone electrophoresis**. Suitable two-phase systems for proteins are difficult to construct, mainly because (a) most prO-teins are easily denatured or precipitated by the organic solvents usually used for preparing phase systems, and (b) proteins are macromolecules and have, therefore, a general tendency to concentrate only in one phase, that is, one obtains either very high or very low partition coefficients. When, as sometimes happens, two proteins concentrate in different phases this is, of course, an advantage since it leads to large separation factors. If, however, two proteins go to the same phase it is difficult to apply partition chromatography or counter-current extraction for their separation.To overcome these difficulties, phase systems have been used which are made by mixing water, salt, and organic solvents soluble in water, like alcohol, glycol ethers, etc.*", in such proportions that the composition is near the plait point. Such systems dissolve many proteins and give finite partition coefficients. Near the plait point the partition coefficient is, however, extremely sensitive to small changes in the composition of the phase system which, in turn, is sensitive to variations in the tem-perature. The composition and temperature must therefore be controlled very carefully. This communication describes some results that have been obtained when proteins are distributed between two-phase systerns composed of water and two high polymers soluble in water. Such systems have been used for the fractionation of cellular particles?. Ref. 8 also contains references to the