AasP autotransporter protein of Actinobacillus pleuropneumoniae does not protect pigs against homologous challenge.
AasP autotransporter protein of Actinobacillus pleuropneumoniae does not protect pigs against homologous challenge.
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DOI：
10.1016/j.vaccine.2009.06.047
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发表时间：
2009-08
期刊：
影响因子：
5.5
通讯作者：
N. Oldfield;Kathryn E. Worrall;A. Rycroft;T. Ali;K. Wooldridge;D. Ala'aldeen
中科院分区：
文献类型：
--
作者：
N. Oldfield;Kathryn E. Worrall;A. Rycroft;T. Ali;K. Wooldridge;D. Ala'aldeen
胸膜肺炎放线杆菌是猪的主要呼吸道病原体；目前的疫苗只能提供有限的保护。 AasP 是一种枯草杆菌蛋白酶样丝氨酸蛋白酶，是一种保守的外膜定位自转运蛋白。我们假设 AasP 会诱导保护性免疫，因此可能构成针对胸膜肺炎放线菌感染的疫苗的有用成分。在这里，我们通过实验证实 AasP 是一种体内表达的抗原蛋白。在猪保护研究中，重组 AasP 诱导了可检测的特异性抗体反应。然而，该疫苗不能保护猪免受同源胸膜肺炎放线菌菌株攻击引起的定植、感染或严重临床疾病。
Actinobacillus pleuropneumoniae is a major respiratory pathogen of pigs; current vaccines provide only limited protection. AasP, a subtilisin-like serine protease, is a conserved outer membrane-localised autotransporter protein. We hypothesized that AasP would induce protective immunity and may thus constitute a useful component of a vaccine against A. pleuropneumoniae infection. Here we confirm experimentally that AasP is an antigenic in vivo-expressed protein. In pig protection studies, a detectable specific antibody response was induced in response to recombinant AasP. However, the vaccine was not capable of protecting pigs from colonization, infection or severe clinical disease resulting from challenge with the homologous A. pleuropneumoniae strain.