Endometrial human chorionic gonadotropin (hCG) expression is a marker for adequate secretory transformation of the endometrium
Endometrial human chorionic gonadotropin (hCG) expression is a marker for adequate secretory transformation of the endometrium
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DOI：
10.1007/s00404-019-05130-y
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发表时间：
2019-06-01
影响因子：
2.6
通讯作者：
Alexander, Henry
中科院分区：
文献类型：
--
作者：
Schug, Sindy;Baunacke, Anja;Alexander, Henry
目的 胚胎成功植入子宫内膜取决于胚胎特征和适当的子宫内膜发育。生殖医学通常关注胚胎质量，但仍然需要对子宫内膜容受性进行可靠的诊断测试。我们之前发现，人类绒毛膜促性腺激素（hCG）是胚胎最早分泌的蛋白质之一，也在着床窗口周围的黄体期子宫内膜中表达。在这里，我们测试了子宫内膜 hCG 作为植入标志物的假设。方法子宫内膜活检和血清样本取自接受常规不孕诊断的患者。分析免疫组织化学检测的子宫内膜 hCG 表达与子宫内膜充分分泌转化、CD45 阳性白细胞浸润、临床诊断参数和子宫内膜厚度的相关性。结果发现，作为正常分泌转化的测量指标的子宫内膜评分与 hCG 染色强度之间存在高度显着相关性。 CD45阳性白细胞的侵袭随着子宫内膜分泌转化的进展和子宫内膜hCG表达的升高而增加。此外，血清孕酮浓度与子宫内膜腺体的 hCG 表达相关。结论我们的结果表明，子宫内膜 hCG 可能作为表征子宫内膜分泌转化的诊断参数，因此也可能表征其着床能力。
Purpose Successful embryo implantation into the endometrium depends on embryonic characteristics and proper endometrial development. Reproductive medicine often focuses on embryo quality, whereas reliable diagnostic tests for endometrial receptivity are still needed. We previously found that human chorionic gonadotropin (hCG), one of the earliest proteins secreted by the embryo, was also expressed by the luteal phase endometrium around the implantation window. Here, we tested our hypothesis of endometrial hCG as an implantation marker.Methods Endometrial biopsies and serum samples were taken from patients undergoing routine infertility diagnostics. Correlations of immunohistochemically detected endometrial hCG expression with adequate endometrial secretory transformation, the infiltration of CD45-positive leukocytes, clinical diagnostic parameters, and endometrial thickness were analyzed.Results A highly significant correlation between the endometrial score, as a measurement for regular secretory transformation, and the intensity of hCG staining was found. The invasion of CD45-positive leukocytes increased with progressing endometrial secretory transformation and rising endometrial hCG expression. In addition, serum progesterone concentrations correlated with hCG expression by the endometrial glands.Conclusions Our results suggest endometrial hCG as a possible diagnostic parameter characterizing the endometrial secretory transformation and, thus, possibly also its implantation capability.