Diversity and distribution of thermophilic hydrogenogenic carboxydotrophs revealed by microbial community analysis in sediments from multiple hydrothermal environments in Japan
Diversity and distribution of thermophilic hydrogenogenic carboxydotrophs revealed by microbial community analysis in sediments from multiple hydrothermal environments in Japan
复制标题
日本多个热液环境沉积物中微生物群落分析揭示嗜热产氢一氧化碳营养菌的多样性和分布
DOI：
10.1007/s00203-019-01661-9
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发表时间：
2019
期刊：
影响因子：
2.8
通讯作者：
Yoshihiko Sako
中科院分区：
文献类型：
--
作者：
Kimiho Omae;Yuto Fukuyama;Hisato Yasuda;Kenta Mise;Takashi Yoshida;Yoshihiko Sako
在热液环境中，嗜热产氢一氧化碳营养菌利用一氧化碳（CO）可以降低CO的毒性水平，并为微生物群落提供H2，从而在微生物生态学中发挥重要作用。我们评估了嗜热产氢一氧化碳微生物群落分析。首先，我们分析了一氧化碳脱氢酶（CODH）-能量转换氢化酶（ECH）基因簇和分类隶属关系之间的相关性，通过调查不断增加的基因组数据库。我们确定了71个基因组编码的CODH-ECH基因簇，包括46个其所有者没有被报道为产氢一氧化碳营养菌。我们鉴定了13个与这些分类群具有> 98.7%同一性的类胡萝卜素类型，作为温泉中潜在的产氢一氧化碳营养菌。其中，厚壁菌门的几种类型如副芽孢杆菌、羧胞菌、卡尔达纳热菌和羧热菌存在于不同的环境条件和不同的微生物群落中。潜在的嗜热产氢一氧化碳营养菌的相对丰度较低。它们中的大多数没有显示出与其他微生物的共生网络，这意味着它们的代谢活动可能很低。
In hydrothermal environments, carbon monoxide (CO) utilisation by thermophilic hydrogenogenic carboxydotrophs may play an important role in microbial ecology by reducing toxic levels of CO and providing H2for fuelling microbial communities. We evaluated thermophilic hydrogenogenic carboxydotrophs by microbial community analysis. First, we analysed the correlation between carbon monoxide dehydrogenase (CODH)–energy-converting hydrogenase (ECH) gene cluster and taxonomic affiliation by surveying an increasing genomic database. We identified 71 genome-encoded CODH–ECH gene clusters, including 46 whose owners were not reported as hydrogenogenic carboxydotrophs. We identified 13 phylotypes showing > 98.7% identity with these taxa as potential hydrogenogenic carboxydotrophs in hot springs. Of these, Firmicutes phylotypes such asParageobacillus,Carboxydocella,Caldanaerobacter,andCarboxydothermuswere found in different environmental conditions and distinct microbial communities. The relative abundance of the potential thermophilic hydrogenogenic carboxydotrophs was low. Most of them did not show any symbiotic networks with other microbes, implying that their metabolic activities might be low.