The involvement of multiple protease-antiprotease systems and gut origin sepsis in zymosan-associated endothelial barrier injury and multiple organ dysfunction in rats
The involvement of multiple protease-antiprotease systems and gut origin sepsis in zymosan-associated endothelial barrier injury and multiple organ dysfunction in rats
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DOI：
10.1097/00024382-200116040-00012
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发表时间：
2001-10-01
期刊：
影响因子：
3.1
通讯作者：
Andersson, R
中科院分区：
文献类型：
--
作者：
Deng, XM;Wang, XD;Andersson, R
多器官功能障碍综合征是重症监护病房死亡的主要原因。实验结果表明，腹腔注射无菌酵素可引起类似多器官功能障碍综合征的症状。在本研究中，我们研究了腹腔注射不同剂量的酶酶san后对多器官功能、内皮通透性和抗蛋白酶的潜在改变。zymosan诱导的全身性炎症导致多个器官/组织的内皮屏障损伤，全身动脉压降低，器官功能和肠道防御功能受损，蛋白酶抑制剂消耗，特别是α(2)抗纤溶酶的消耗。内皮屏障损伤在多个器官/组织中表现出剂量和器官依赖的模式，内皮屏障通透性的增加发生在器官功能障碍之前。Zymosan诱导多器官功能障碍综合征的发展，可能启动多种蛋白酶-抗蛋白酶系统，特别是纤维蛋白溶解系统，导致内皮屏障损伤、组织水肿、实质细胞损伤和最终器官功能障碍，并可能因继发性细菌感染而加剧。
Multiple organ dysfunction syndrome is a dominant cause of mortality in the intensive care unit. Experimentally, a condition similar to the multiple organ dysfunction syndrome can be induced by the intraperitoneal injection of sterile zymosan. In the present study we investigate potential alterations in multiple organ functions, endothelial permeability, and antiproteinases after intraperitoneal injection of zymosan at various doses. Zymosan-induced generalized inflammation lead to endothelial barrier injury in multiple organs/tissues, a decrease in systemic arterial pressure, impaired organ function and gut defence function, and consumption of protease inhibitors, particularly the consumption of alpha (2) antiplasmin. Endothelial barrier injury appears to present a dose- and organ-dependent pattern in multiple organs/tissues, and the increase in endothelial barrier permeability occurred prior to organ dysfunction. Zymosan induced the development of multiple organ dysfunction syndrome, probably initiating multiple protease-antiprotease systems, particularly the fibrinolytic system, leading to endothelial barrier injury, tissue edema, parenchymal cell damage, and eventual organ dysfunction, potentially augmented by a secondary bacterial infection.