Allelic forms of anti-phosphorylcholine antibodies.
Allelic forms of anti-phosphorylcholine antibodies.
复制标题
抗磷酸胆碱抗体的等位基因形式。
作者：
R. Lieberman;S. Rudikoff;W. Humphrey;M. Potter
制备了2种磷酸胆碱（PC）结合骨髓瘤蛋白（BALB/c来源的T15和C57 BL来源的C3）的抗独特型抗血清。显示这些抗血清识别2个亲本菌株中诱导的抗PC抗体上的相应决定簇。在（BALB/c × C57 BL/6）× BALB/c回交后代中对这两个V区标记的遗传分析表明，这两个决定簇以简单的孟德尔等位基因形式与同种异型位点连锁。遗传数据与先前报道的结构研究一致，表明T15和C3重链可变区的等位基因性质。T15和C3抗原决定簇在来自BALB/c-C57 BL/6杂交的IG同源和重组近交系中的表达进一步支持等位性的概念，尽管观察到一个例外。菌株分布分析表明，除了C3之外，T15基因的其他等位基因的潜在存在。
Anti-idiotype antisera were prepared to 2 phosphorylcholine (PC) binding myeloma proteins, T15 of BALB/c origin and C3 of C57BL origin. These antisera were shown to recognize the respective determinants on induced anti-PC antibodies in the 2 parental strains. Genetic analysis of these V region markers in backcross progeny of (BALB/c X C57BL/6) X BALB/c demonstrated that the 2 determinants segregated as simple Mendelian alleles linked to the allotype locus. The genetic data are consistent with previously reported structural studies suggesting the allelic nature of the T15 and C3 heavy chain variable regions. The expression of the T15 and C3 antigenic determinants in Ig congenic and recombinant inbred strains derived from BALB/c-C57BL/6 crosses further supports the concept of allelism although a single exception was observed. Strain distribution analysis has indicated the potential existence of other alleles of the T15 gene in addition to C3.