Measurement of ATP in Single Oocytes: Impact of Maturation and Cumulus Cells on Levels and Consumption
Measurement of ATP in Single Oocytes: Impact of Maturation and Cumulus Cells on Levels and Consumption
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DOI：
10.1002/jcp.24457
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发表时间：
2014-03-01
影响因子：
5.6
通讯作者：
Carroll, John
中科院分区：
文献类型：
--
作者：
Dalton, Caroline M.;Szabadkai, Gyorgy;Carroll, John
线粒体是卵母细胞和早期胚胎中 ATP 的主要来源，线粒体功能障碍和线粒体来源 ATP 的缺乏与发育能力欠佳有关。我们使用新型重组 FRET 探针 AT1.03 对成熟小鼠卵母细胞中的 ATP 进行了研究。我们证明 AT1.03 可成功用于长时间监测单个活卵母细胞中的胞质 ATP 水平。我们发现 ATP 水平经历与特定成熟事件相关的动态变化，并且卵母细胞在成熟的不同阶段显示出不同的 ATP 消耗率。卵丘封闭的卵母细胞在成熟过程中比裸露的卵母细胞具有更高的 ATP 水平，并且可以通过抑制卵母细胞和卵丘细胞之间的间隙连接通讯来消除这种情况。我们的工作使用一种新方法来阐明卵母细胞成熟过程中 ATP 水平和 ATP 消耗的调节。 J.细胞。生理学。 229：353-361，2014 年。(c) 2013 年 Wiley 期刊公司。
Mitochondria provide the primary source of ATP in the oocyte and early embryo and mitochondrial dysfunction and deficit of mitochondria-derived ATP has been linked to suboptimal developmental competence. We have undertaken a study of ATP in the maturing mouse oocyte using a novel recombinant FRET based probe, AT1.03. We show that AT1.03 can be successfully used to monitor cytosolic ATP levels in single live oocytes over extended time periods. We find that ATP levels undergo dynamic changes associated with specific maturational events and that oocytes display altered rates of ATP consumption at different stages of maturation. Cumulus enclosed oocytes have a higher ATP level during maturation than denuded oocytes and this can be abolished by inhibition of gap junctional communication between the oocyte and cumulus cells. Our work uses a new approach to shed light on regulation of ATP levels and ATP consumption during oocyte maturation. J. Cell. Physiol. 229: 353-361, 2014. (c) 2013 Wiley Periodicals, Inc.