Irreversible magnetoporation of micro-organisms in high pulsed magnetic fields
Irreversible magnetoporation of micro-organisms in high pulsed magnetic fields
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DOI：
10.1049/iet-nbt.2013.0005
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发表时间：
2014-09-01
影响因子：
2.3
通讯作者：
Markovskaja, Svetlana
中科院分区：
文献类型：
--
作者：
Novickij, Vitalij;Grainys, Audrius;Markovskaja, Svetlana
电穿孔是一种刺激活细胞的吸引人的方式，其在生物对象的结构中引起永久或暂时的纳米孔隙。然而，该技术具有缺点，例如需要电极和细胞培养基之间的接触。在这篇综述中，提出了一种利用脉冲磁场对生物体进行非接触式处理的方法。真核微生物Achlya americana和Saprolegnia diclina已被用于研究中，并施加高达7 T的磁场，其引起类似于导致物种死亡的不可逆电穿孔的效应。所提出的技术适用于不同类型的生物细胞或微生物，并且可能可用于癌症、抗真菌治疗和其他生物技术领域。
Electroporation is an appealing way of stimulating living cells, which causes permanent or temporary nanoporosities in the structure of the biological objects. However, the technique has a disadvantage such as a requirement of contact between the electrodes and the cell medium. In this review, a methodology of contactless treatment of the biological objects using pulsed magnetic fields is proposed. The eukaryotic micro-organisms Achlya americana and Saprolegnia diclina have been used in the study and magnetic fields up to 7 T were applied, which caused effects similar to irreversible electroporation resulting in the death of the species. The proposed technique is applicable for different types of the biological cells or micro-organisms and possibly can be used in the area of cancer, antifungal treatment and other biotechnological fields.