Antiandrogenic activity of Calotropis procera latex in rats.
Antiandrogenic activity of Calotropis procera latex in rats.
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DOI：
10.4103/2305-0500.233574
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发表时间：
2018-01-01
影响因子：
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通讯作者：
Elsheikh, A. S.
中科院分区：
文献类型：
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作者：
Abdelgader, A. B.;Elsheikh, A. S.
目的：探讨马蹄莲乳汁对未成年雄性Wistar大鼠青春期发育的影响。研究方法：将30只3周龄未成年雄性Wistar大鼠随机分为5组：A组（对照组），蒸馏水作为安慰剂; B组，每天口服马蹄莲胶乳混悬液，剂量率为5 mg/kg体重（BW），C组为10 mg/kg BW; D组为15 mg/kg BW，E组为20 mg/kg BW。每日称量大鼠体重以调整剂量并记录BW变化，并继续给药4周;此后处死大鼠。从所有大鼠中采集血清样品（n=30），冷冻保存，直至测定生殖激素。此外，对睾丸和附睾进行称重;对附睾精子进行计数;估计精子活力和异常;并制备睾丸的组织病理学切片。结果如下：结果表明，幼龄雄性大鼠经口灌服10 mg/kg体重的马唐胶乳，对大鼠的体重有显著的促进作用（P
Objective: To determine the effects of Calotropis procera latex on pubertal traits of immature male Wistar rats. Methods: A total of 30 immature male Wistar rats aged 3 weeks old were grouped randomly into 5 groups: group A (control group) was offered distilled water as a placebo; group B was daily oral dosed with suspension of Calotropis procera latex at a dose rate of 5 mg/kg body weight (BW), group C 10 mg/kg BW; group D 15 mg/kg BW and group E 20 mg/kg BW. The rats were weighed daily to adjust the dose and record the BW changes, and the treatment continued for 4 weeks; thereafter, rats were sacrificed. Serum samples (n=30) were collected from all rats and kept frozen until assayed for reproductive hormones. Furthermore, the testes and epididymae were weighed; epididymal sperms were counted; sperm motility & abnormality were estimated; and histopathological sections of the testes were prepared. Results: The results of this study showed that oral dosing of immature male rats with Calotropis procera latex at doses rate 10 mg/kg BW significantly (P