Restricted localization of thrombospondin-2 protein during mouse embryogenesis: a comparison to thrombospondin-1.
Restricted localization of thrombospondin-2 protein during mouse embryogenesis: a comparison to thrombospondin-1.
复制标题
小鼠胚胎发生过程中血小板反应蛋白 2 蛋白的限制性定位：与血小板反应蛋白 1 的比较。
DOI：
10.1016/s0945-053x(98)90026-9
复制
发表时间：
1998
期刊：
影响因子：
--
通讯作者：
Mosher,DF
中科院分区：
文献类型：
--
作者：
Tooney,PA;Sakai,T;Sakai,K;Aeschlimann,D;Mosher,DF
Thrombospondin-1 和 -2（TSP1 和 TSP2）是由不同基因编码的多功能、多模块细胞外基质蛋白。我们通过免疫组织化学比较了小鼠胚胎（第 10 天及以后）中 TSP1 和 TSP2 的分布。第 10 天在心脏和肠上皮中检测到 TSP1，第 11 天在巨核细胞中检测到 TSP1，第 14 天在肺中检测到。 TSP2 直到第 14 天才被检测到，在产生软骨和骨的间充质凝结中染色最强。 TSP2的分布与TSP1的分布不同但有重叠。 TSP1 存在于软骨本身中，在分化和肥大的软骨细胞周围染色减少，而 TSP2 仅限于生长区软骨的软骨细胞周围的基质。 TSP2和TSP1均在形成颅骨的膜内骨化中心、网状真皮、鼻上皮顶面、骨骼肌和触毛周围的鞘中表达。 TSP2 的专属染色区域包括鼻腔软骨周围的软骨膜、下颌骨的发育骨和肾上腺。 TSP1 和 TSP2 的不同定位表明这两种蛋白在小鼠胚胎发生过程中具有特定的功能。
Thrombospondin-1 and -2 (TSP1 and TSP2) are multifunctional, multimodular extracellular matrix proteins encoded by separate genes. We compared the distributions of TSP1 and TSP2 in mouse embryos (day 10 and later) by immunohistochemistry. TSP1 was detected on day 10 in the heart and intestinal epithelium, on day 11 in megakaryocytes, and on day 14 in the lung. TSP2 was not detected until day 14, with strongest staining in mesenchymal condensation that gives rise to cartilage and bone. The distribution of TSP2 was different from but overlapped with the distribution of TSP1. TSP1 was found in cartilage proper with diminished staining around chondrocytes undergoing differentiation and hypertrophy, whereas TSP2 was restricted to the matrix surrounding chondrocytes of the growth zone cartilage. TSP2 and TSP1 were both expressed in centers of intramembranous ossification that form the skull bones, in reticular dermis, on the apical surface of nasal epithelium, in skeletal muscle, and in the sheath surrounding vibrissae. Areas of exclusive staining for TSP2 included the perichondrium surrounding the cartilage of the nasal cavities, developing bone of the lower mandible, and adrenal gland. The distinct localizations of TSP1 and TSP2 indicate that the two proteins have specific functions during mouse embryogenesis.