Availability of short microsatellite markers from butterfly museum and private specimens
Availability of short microsatellite markers from butterfly museum and private specimens
复制标题
蝴蝶博物馆和私人标本的短微卫星标记的可用性
DOI：
10.1111/ens.12215
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发表时间：
2017
影响因子：
0.9
通讯作者：
Yuji Isagi
中科院分区：
文献类型：
--
作者：
Naoyuki Nakahama;Yuji Isagi
了解遗传多样性和遗传结构的时间变化对于确定导致濒危昆虫物种减少的因素以及建立这些物种的保护计划是重要的。因此，最近人们对利用历史博物馆标本的DNA数据恢复遗传多样性的保护计划越来越感兴趣。在蝴蝶标本中，我们测量了提取DNA的产量和片段大小，并研究了9个短微卫星标记（等位基因大小为73-191 bp）的基因分型成功率。我们使用了20世纪60年代至2010年代收集的中型蝴蝶Melitaea ambigua（鳞翅目；蛱蝶科）标本的腿部样本。超过150 bp的DNA提取率随着样品年龄的增加而降低。所有微卫星标记的基因分型成功率与标本年龄呈负相关。各微卫星标记的等位基因大小与基因分型成功率呈负相关。我们认为，短微卫星标记和最近获得的标本分析特别适合于蝴蝶标本的微卫星分析。
Knowledge of the temporal changes in genetic diversity and structure is important for identifying factors causing a decline in threatened insect species, and for establishing conservation programs for these species. Thus, there is recently an increasing interest in the restoration of genetic diversity in conservation programs using DNA data from historical museum specimens. For butterfly specimens, we measured the yields and fragment sizes of the extracted DNA and investigated the genotyping success probability of nine short microsatellite markers (allele size 73–191 bp). We used leg samples of specimens of a medium‐sized butterfly species,Melitaea ambigua(Lepidoptera; Nymphalidae), collected from the 1960s to the 2010s. The yields of specimen‐extracted DNA longer than 150 bp decreased with increasing specimen age. There were negative correlations between the genotyping success probability and specimen age for each of all microsatellite markers. A negative correlation was also observed between the genotyping success probability and allele size of each microsatellite marker. We conclude that short microsatellite markers and analysis of recently obtained specimens are particularly suitable for microsatellite analysis of butterfly specimens.