Expression of stathmin in human uterus and decidualizing endometrial stromal cells.
Expression of stathmin in human uterus and decidualizing endometrial stromal cells.
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DOI：
10.1530/rep.1.01148
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发表时间：
2006-10
期刊：
影响因子：
3.8
通讯作者：
K. Tamura;M. Yoshie;H. Nishi;Yumi Osakabe;K. Isaka;T. Hara;H. Kogo
中科院分区：
文献类型：
--
作者：
K. Tamura;M. Yoshie;H. Nishi;Yumi Osakabe;K. Isaka;T. Hara;H. Kogo
啮齿类动物胚胎着床部位胞浆磷蛋白stathmin表达上调。然而，stathmin在人子宫中的表达尚未被研究。采用免疫组化法检测子宫和胎盘组织stathmin的分布，逆转录聚合酶链反应（RT-PCR）检测子宫内膜组织stathmin mRNA的表达。培养的子宫内膜间质细胞被用来研究stathmin是否在蜕膜化中发挥作用。Stathmin在人子宫内膜组织的腺上皮和基质细胞中特异性表达。在妊娠早期，它也被细胞滋养层和绒毛外滋养层表达，但不被合体滋养层或蜕膜组织表达。当从正常子宫内膜组织中分离的基质细胞培养并用孕酮（P4）加雌激素或双丁酰环3 '，5'-AMP刺激蜕膜化时，其总stathmin和磷酸化stathmin水平降低。敲低stathmin在培养的基质细胞中的表达使用小干扰RNA，细胞暴露于蜕膜化剂之前，显着抑制蜕膜化，如IGF结合蛋白-1和催乳素的表达减少所示。Stathmin在人子宫内膜和胎盘细胞中表达不同，可能参与子宫内膜间质细胞的蜕膜化。
The cytosolic phosphoprotein stathmin is upregulated at the site of embryo implantation in the rodents. However, stathmin expression in the human uterus has not yet been investigated. The distribution of uterine and placental stathmin was analyzed by immunohistochemistry, while stathmin mRNA expression was detected in endometrial tissues by the reverse transcriptase-PCR. Cultured endometrial stromal cells were used to investigate whether stathmin plays a role in decidualization. Stathmin is expressed specifically in the glandular epithelium and the stromal cells of human endometrial tissue. It is also expressed by cytotrophoblasts and extravillous trophoblasts, but not by syncytiotrophoblasts or decidual tissues during the first trimester of pregnancy. When stromal cells isolated from normal endometrial tissues were cultured and stimulated to decidualize by progesterone (P4) plus estrogen or dibutyryl cyclic 3',5'-AMP, their total and phosphorylated stathmin levels decreased. Knocking down stathmin expression in the cultured stromal cells using small interfering RNA, before the cells were exposed to the decidualizing agents, significantly suppressed decidualization, as indicated by the decreased expression of IGF-binding protein-1 and prolactin. Stathmin is differently expressed in human endometrial and placental cells and may participate in the decidualization of endometrial stromal cells.