Characterization the role of GacA-dependent small RNAs and RsmA family proteins on 2,4-diacetylphloroglucinol production in Pseudomonas fluorescens 2P24
Characterization the role of GacA-dependent small RNAs and RsmA family proteins on 2,4-diacetylphloroglucinol production in Pseudomonas fluorescens 2P24
复制标题
表征 GacA 依赖性小 RNA 和 RsmA 家族蛋白对荧光假单胞菌 2P24 中 2,4-二乙酰基间苯三酚生产的作用
DOI：
10.1016/j.micres.2019.126391
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发表时间：
2020-03-01
影响因子：
6.7
通讯作者：
Zhang, Li-Qun
中科院分区：
文献类型：
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作者：
Zhang, Yang;Zhang, Bo;Zhang, Li-Qun
荧光假单胞菌 2P24 是一种对植物有益的根际细菌，可控制许多由土传病原体引起的根部疾病，而抗生素化合物 2,4-二乙酰基间苯三酚 (2,4-DAPG) 的产生对其生物防治能力至关重要。在本研究中，我们研究了菌株 2P24 中 GacA 依赖性小非编码 RNA (sRNA) 和 RsmA/E 蛋白对 2,4-DAPG 产生的调节机制。我们的结果表明，GacS-GacA 系统通过诱导 rsmX、rsmX1、rsmY 和 rsmZ 的表达来调节负责 2,4-DAPG 生产的 phlACBD 基因座的表达。一种新型 GacA 调节的 sRNA RgsA 被发现可以负向调节 2,4-DAPG 的产生。 GacS-GacA 系统对 phlACBD 基因座的激活是通过 RsmA 和 RsmE 蛋白（但不是 RsmI）介导的，它们通过与 phlACBD 前导序列中假定的 RsmA/E 识别元件结合来抑制 phlACBD 翻译。综上所述，我们的结果表明，在荧光假单胞菌 2P24 中，GacS-GacA 系统通过微调荧光假单胞菌中 sRNA 的功能来控制细胞中的 2,4-DAPG 水平。
Pseudomonas fluorescens 2P24 is a plant-beneficial rhizobacteria that controls many root diseases caused by soilborne pathogens, and the production of the antibiotic compound 2,4-diacetylphloroglucinol (2,4-DAPG) is essential for its biocontrol ability. In the present study, we investigated the regulatory mechanism acting on the production of 2,4-DAPG by the GacA-dependent small non-coding RNAs (sRNAs) and RsmA/E proteins in strain 2P24. Our results showed that the GacS-GacA system regulates the expression of the phlACBD locus, which is responsible for 2,4-DAPG production, by inducing the expression of rsmX, rsmX1, rsmY, and rsmZ. A novel GacA-regulated sRNA, RgsA, was found to negatively regulate 2,4-DAPG production. Activation of the phlACBD locus by the GacS-GacA system is mediated through RsmA and RsmE proteins (but not RsmI), which inhibit phlACBD translation by binding to the putative RsmA/E recognition element in the phlACBD leader. Taken together, our results suggested that in P. fluorescens 2P24, the GacS-GacA system controls the cellular 2,4-DAPG levels in the cell by fine-tuning the function of sRNAs in P. fluorescens.