The influence of the route of administration of gold nanoparticles on their tissue distribution and basic biochemical parameters: In vivo studies
The influence of the route of administration of gold nanoparticles on their tissue distribution and basic biochemical parameters: In vivo studies
复制标题
DOI：
10.1016/j.pharep.2014.10.019
复制
发表时间：
2015-06-01
影响因子：
4.4
通讯作者：
Tesiorowski, Maciej
中科院分区：
文献类型：
--
作者：
Bednarski, Marek;Dudek, Magdalena;Tesiorowski, Maciej
背景资料：金纳米颗粒（AuNP）表现出有趣的化学和物理性质，因此在医学中进行了深入的测试。然而，缺乏关于这些纳米颗粒的毒性以及它们从体内排泄的信息。因此，本研究的目的是调查的金纳米粒子给药途径的影响，其在组织中的分布和排泄率。方法：实验进行了雄性Wistar大鼠。每1kg体重给予每毫升含0.3619mg颗粒的胶体金混悬液。测定动物血清丙氨酸氨基转移酶、天冬氨酸氨基转移酶、总胆固醇、甘油三酯、葡萄糖和血浆铁还原能力。此外，还检查了po或iv给药后连续几天内的金属排泄。此外，研究了静脉内施用后3天AuNP对一些生化参数的影响。结果表明，金纳米颗粒主要蓄积在肝，肺和脾静脉给药后，只有少量从体内的尿液和粪便中去除。结论：这些纳米颗粒的积累效果，在FRAP和葡萄糖水平的增加高达27%和73%，分别。这反过来表明AuNP的静脉内施用可能影响严重的医学并发症。在另一个部位，大约50%的引入到大鼠体内的颗粒在肝脏中的积累对于光疗是有希望的，并且它为药物转运到该器官打开了“门”。(C)2014年由Elsevier Sp. z o.o.发布代表波兰科学院药理学研究所。
Background: The gold nanoparticles (AuNP's) exhibit interesting chemical and physical properties and for this reason are intensively tested in medicine. However there is a lack of information about toxicity of those nanoparticles as well as their excretion from the body. Thus, the aim of the present study was to investigate the influence of the route of administration of gold nanoparticles to rats on their distribution in tissues and excretion rate.Methods: The experiment was carried out on male Wistar rats. The colloidal gold suspension containing 0.3619 mg of particles per milliliter, was administered per 1 kg of body weight. Serum levels of alanine aminotransferase, aspartate aminotransferase, total cholesterol, triglycerides, glucose and ferric reducing ability of plasma were measured in all investigated animals.Results: It was shown that after oral administration only a small amount of AuNPs was absorbed. In addition, excretion of the metal during consecutive days after po or iv administration was examined. Moreover, the impact of AuNPs on some biochemical parameters 3 days after intravenous administration was studied. It was shown that the AuNPs are mainly cumulated in the liver, lungs and in spleen after iv administration and only slightly removed from the body in urine and feces.Conclusion: Accumulation of those nanoparticles effect in increases of FRAP and glucose level up to 27% and 73%, respectively. This in turn suggests that iv administration of AuNPs may effect in serious medical complications. On the other site, the accumulation in the liver of about 50% of introduced particles to the rats body is promising for phototherapy and it opens "door" for drug transport to this organ. (C) 2014 Published by Elsevier Sp. z o.o. on behalf of Institute of Pharmacology, Polish Academy of Sciences.