Mouse Model for Optogenetic Genome Engineering.
Mouse Model for Optogenetic Genome Engineering.
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DOI：
10.18926/amo/63202
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发表时间：
2022-02
影响因子：
0.5
通讯作者：
Tomoka Takao;D. Yamada;T. Takarada
中科院分区：
文献类型：
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作者：
Tomoka Takao;D. Yamada;T. Takarada
光遗传学是一种通过光来操纵生物现象的技术，在神经科学领域引起了极大的关注。最近，一种光诱导的蛋白质二聚化系统Magnet系统被开发出来，它可以利用光高精度地控制各种生物分子的细胞内行为。此外，通过将该技术与基因组编辑技术(CRISPR/Cas9系统)或DNA重组技术(CRE-loxP系统)相结合，正在开发用于控制生物现象的光激活系统。在这里，我们回顾了光遗传学和最新的磁力系统技术的历史，并介绍了我们最近开发的具有时间、空间和细胞特异性的光激活Cre敲入小鼠。
Optogenetics, a technology to manipulate biological phenomena thorough light, has attracted much attention in neuroscience. Recently, the Magnet System, a photo-inducible protein dimerization system which can control the intracellular behavior of various biomolecules with high accuracy using light was developed. Furthermore, photoactivation systems for controlling biological phenomena are being developed by combining this technique with genome-editing technology (CRISPR/Cas9 System) or DNA recombination technology (Cre-loxP system). Herein, we review the history of optogenetics and the latest Magnet System technology and introduce our recently developed photoactivatable Cre knock-in mice with temporal-, spatial-, and cell-specific accuracy.