Molecular vibrational imaging of a human cell by multiplex coherent anti-Stokes Raman scattering microspectroscopy using a supercontinuum light source
Molecular vibrational imaging of a human cell by multiplex coherent anti-Stokes Raman scattering microspectroscopy using a supercontinuum light source
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DOI：
10.1002/jrs.2041
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发表时间：
2008-11-01
影响因子：
2.5
通讯作者：
Kano, Hideaki
中科院分区：
文献类型：
--
作者：
Kano, Hideaki
利用多重相干反斯托克斯拉曼散射（汽车）显微光谱技术，获得了人体细胞的分子振动图像。HeLa细胞在C-H伸缩振动模式下的汽车图像清楚地显示了各种细胞器，如核仁，染色体，细胞膜和核膜，具有高振动对比度。在中间相中也观察到了丝状结构，而在M相中观察不到。这些结构最可能可视化内质网（ER）网络。版权所有（C）2008约翰威利父子有限公司
Molecular vibrational images of human cells are obtained by multiplex coherent anti-Stokes Raman scattering (CARS) microspectroscopy. The CARS images of HeLa cells at the C-H stretching vibrational mode clearly show various organelles such as nucleolus, chromosome, cell membrane, and nuclear membrane with high vibrational contrast. Filament-like structures are also found to be observed in interphase, while it is not clearly observed in M phase. These structures most probably visualize endoplasmic reticulum (ER) network. Copyright (C) 2008 John Wiley & Sons, Ltd.