Identification of differentially expressed genes in mouse hepatocarcinoma ascites cell line with low potential of lymphogenous metastasis
Identification of differentially expressed genes in mouse hepatocarcinoma ascites cell line with low potential of lymphogenous metastasis
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DOI：
10.3748/wjg.v12.i42.6893
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发表时间：
2006-11-14
影响因子：
4.3
通讯作者：
Ban, Li-Ying
中科院分区：
文献类型：
--
作者：
Cui, Xiao-Nan;Tang, Jian-Wu;Ban, Li-Ying
目的：鉴定低淋巴转移潜能小鼠肝癌腹水细胞系中差异表达的基因。方法：采用抑制消减杂交（SSH）方法构建低淋巴转移潜能小鼠肝癌腹水细胞系Hca-P及其高转移潜能同系细胞系Hca-F的消减cDNA文库。对筛选出的消减文库克隆进行测序，并进行GenBank同源性检索。结果：获得15个Hca-P差异表达cDNA片段，其中已知基因8个，表达序列标签（EST）4个，3个cDNA无同源性。结论：肿瘤转移是一个涉及多个基因的事件。 SSH是检测差异表达基因的有用技术，也是克隆新基因的有效方法。 (C) 2006 年 WJG 出版社。版权所有。
AIM: To identify genes differentially expressed in mouse hepatocarcinoma ascites cell line with low potential of lymphogenous metastasis.METHODS: A subtracted cDNA library of mouse hepatocarcinoma cell line with low potential of lymphogenous metastasis Hca-P and its synogenetic cell line Hca-F with high metastatic potential was constructed by suppression subtracted hybridization (SSH) method. The screened clones of the subtracted library were sequenced and GenBank homology search was performed.RESULTS: Fifteen differentially expressed cDNA fragments of Hca-P were obtained which revealed 8 known genes, 4 expressed sequence tags (ESTs) and 3 cDNAs showed no homology.CONCLUSION: Tumor metastasis is an incident involving multiple genes. SSH is a useful technique to detect differentially expressed genes and an effective method to clone novel genes. (C) 2006 The WJG Press. All rights reserved.