Pantothenase-based assay of pantothenic acid.
Pantothenase-based assay of pantothenic acid.
复制标题
基于泛酸酶的泛酸测定。
DOI：
10.1016/0003-2697(83)90272-5
复制
发表时间：
1983
影响因子：
--
通讯作者：
R. Airas
中科院分区：
文献类型：
--
作者：
R. Airas
Pantothenic acid from various sources can be measured in concentrations from about 10 μm to 20 mm by using a specific enzyme, pantothenase. The method is based on the transfer of [14C]β-alanine to the pantothenate via an acyl-enzyme intermediate (a β-alanine-exchange reaction): Although the reaction is inhibited by some compounds, the method can be used for the assay of pantothenate in many biological samples if an internal pantothenate standard is used.
