Clinical outcomes of transfer of frozen and thawed single blastocysts derived from nonpronuclear and monopronuclear zygotes
Clinical outcomes of transfer of frozen and thawed single blastocysts derived from nonpronuclear and monopronuclear zygotes
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DOI：
10.1002/rmb2.12275
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发表时间：
2019-07-01
影响因子：
3.4
通讯作者：
Shimizu, Yasufumi
中科院分区：
文献类型：
--
作者：
Hondo, Saki;Arichi, Akane;Shimizu, Yasufumi
目的在辅助生殖技术中，正常受精卵在受精确认时为双原核(2PN)，但在常规观察中有时会发现非原核受精卵(0PN)和单核受精卵(1PN)。方法为了阐明体外受精胚胎的临床应用价值，我们在日本神奈川市Denentoshi妇科诊所对冷冻解冻的1PN和0PN来源的单个胚泡移植后的临床妊娠率、活产率、流产率和先天畸形进行了调查。结果常规体外受精获得的1PN来源的囊胚妊娠率和活产率分别为37.5%和27.1%，与2PN来源的囊胚相比差异无统计学意义，但0PN来源的囊胚率显著低于2PN来源的囊胚率。卵胞浆内单精子注射(ICSI)获得的胚胎妊娠率和活产率分别为45.7%和34.8%，与2PN来源的囊胚相比差异不显著，但1PN来源的囊胚率(4.0%)显著低于2PN来源的囊胚和0PN来源的囊胚。在移植0PN和1PN来源的胚泡的婴儿中，没有发现先天性异常。结论1PN和0PN来源的胚泡均可用于胚胎移植，但1PN来源的胚泡，尤其是通过ICSI获得的胚泡，应慎重考虑。
Purpose In assisted reproductive technology, normal zygotes are bipronuclear (2PN) during fertilization confirmation; however, sometimes, nonpronuclear zygotes (0PN) and monopronuclear zygotes (1PN) are found during routine observations. Methods To elucidate the clinical usefulness of in vitro-fertilized embryos, we investigated the rates of clinical pregnancy, live birth, miscarriage, and congenital abnormality after transfer of frozen-thawed 1PN- and 0PN-derived single blastocysts at Denentoshi Ladies Clinic, Kanagawa, Japan. Results The rates of pregnancy and live birth for 1PN-derived blastocysts obtained by conventional in vitro fertilization were 37.5% and 27.1%, respectively, which was not significantly different from those for 2PN-derived blastocysts; however, the rates for 0PN-derived blastocysts were significantly lower. The pregnancy and live birth rates for 0PN-derived embryos obtained by intracytoplasmic sperm injection (ICSI) were 45.7% and 34.8%, respectively, which was not significantly different from those for 2PN-derived blastocysts; however, the rates for 1PN-derived blastocysts were significantly lower (4.0% for both) than those for 2PN- and 0PN-derived blastocysts. No congenital abnormalities were found in infants resulting from transfer of 0PN- or 1PN-derived blastocysts. Conclusions Both 1PN- and 0PN-derived blastocysts can be used for embryo transfer; however, care should be taken in making decisions about 1PN-derived blastocysts, especially if they are obtained by ICSI.