The rice wound-inducible transcription factor RERJ1 sharing same signal transduction pathway with OsMYC2 is necessary for defense response to herbivory and bacterial blight
The rice wound-inducible transcription factor RERJ1 sharing same signal transduction pathway with OsMYC2 is necessary for defense response to herbivory and bacterial blight
复制标题
DOI：
10.1007/s11103-021-01186-0
复制
发表时间：
2021-09
影响因子：
5.1
通讯作者：
I. Valea;A. Motegi;N. Kawamura;Koichi Kawamoto;A. Miyao;R. Ozawa;J. Takabayashi;K. Gomi;
中科院分区：
文献类型：
--
作者：
I. Valea;A. Motegi;N. Kawamura;Koichi Kawamoto;A. Miyao;R. Ozawa;J. Takabayashi;K. Gomi;
关键信息本研究描述了bHLH转录因子RERJ 1的生物学功能，涉及茉莉酸响应和相关的防御相关的代谢途径在水稻中，特别侧重于破译潜在的压力诱导的挥发性排放和植食性抗性的调节机制。RERJ 1可被创伤和茉莉酸盐处理迅速而强烈地诱导，但其生物学功能尚不清楚。在这里，我们提供了RERJ 1在植物防御中的生物学功能的证据，特别是在响应植食性和病原体攻击中，并提供了RERJ 1介导的调节专门防御化合物的代谢途径的见解，如单萜芳樟醇，可能与OsMYC 2-一个众所周知的茉莉酸信号传导的主调节因子合作。碱性螺旋-环-螺旋（bHLH）家族转录因子RERJ 1在环境胁迫（如创伤和干旱）下被诱导，这与茉莉酸（JA）积累密切相关。在这里，我们调查的生物学功能的RERJ 1响应生物胁迫，如草食动物和病原体感染，使用一个RERJ 1缺陷突变体。对therj 1-Tos 17突变体的转录组分析显示，RERJ 1调节一个典型的保守的JA应答基因家族的表达（例如，萜烯脱氢酶、蛋白酶抑制剂和茉莉酮酸酯ZIM结构域蛋白）。在粘虫的攻击下，therj 1-Tos 17突变体表现出比野生型更严重的损害，并且观察到以突变体喂养的幼虫的体重显著增加。结果表明，突变体j1-Tos 17在感染水稻黄单胞菌后症状加重。在RERJ 1调控的萜烯脱氢酶中，芳樟醇合成酶的表达被显著阻断，芳樟醇的释放量显著降低; RERJ 1与JA信号的主要调控因子OsMYC 2和许多OsJAZ蛋白相互作用，但在转录水平上没有检测到明显的上位性相互作用。这些结果表明，RERJ 1通过与OsMYC 2的物理结合参与JA介导的胁迫应答基因的转录诱导，并通过JA信号介导防御植物和细菌感染。
Key messageThis study describes biological functions of the bHLH transcription factor RERJ1 involved in the jasmonate response and the related defense-associated metabolic pathways in rice, with particular focus on deciphering the regulatory mechanisms underlying stress-induced volatile emission and herbivory resistance. RERJ1 is rapidly and drastically induced by wounding and jasmonate treatment but its biological function remains unknown as yet. Here we provide evidence of the biological function of RERJ1 in plant defense, specifically in response to herbivory and pathogen attack, and offer insights into the RERJ1-mediated regulation of metabolic pathways of specialized defense compounds, such as monoterpene linalool, in possible collaboration with OsMYC2—a well-known master regulator in jasmonate signaling.AbstractIn rice (Oryza sativaL.), the basic helix–loop–helix (bHLH) family transcription factorRERJ1is induced under environmental stresses, such as wounding and drought, which are closely linked to jasmonate (JA) accumulation. Here, we investigated the biological function ofRERJ1in response to biotic stresses, such as herbivory and pathogen infection, using anRERJ1-defective mutant. Transcriptome analysis of thererj1-Tos17mutant revealed thatRERJ1regulated the expression of a typical family of conserved JA-responsive genes (e.g., terpene synthases, proteinase inhibitors, and jasmonate ZIM domain proteins). Upon exposure to armyworm attack, thererj1-Tos17mutant exhibited more severe damage than the wildtype, and significant weight gain of the larvae fed on the mutant was observed. UponXanthomonas oryzaeinfection, thererj1-Tos17mutant developed more severe symptoms than the wildtype. AmongRERJ1-regulated terpene synthases, linalool synthase expression was markedly disrupted and linalool emission after wounding was significantly decreased in thererj1-Tos17mutant.RERJ1appears to interact withOsMYC2—a master regulator of JA signaling—and many OsJAZ proteins, although no obvious epistatic interaction was detected between them at the transcriptional level. These results indicate thatRERJ1is involved in the transcriptional induction of JA-mediated stress-responsive genes via physical association withOsMYC2and mediates defense against herbivory and bacterial infection through JA signaling.