Comparative analysis of gnathostome Otx gene expression patterns in the developing eye: implications for the functional evolution of the multigene family
Comparative analysis of gnathostome Otx gene expression patterns in the developing eye: implications for the functional evolution of the multigene family
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DOI：
10.1016/j.ydbio.2004.11.019
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发表时间：
2005-02-15
影响因子：
2.7
通讯作者：
Saule, S
中科院分区：
文献类型：
--
作者：
Plouhinec, JL;Leconte, L;Saule, S
我们已经进行了详细的分析，以获得新的见解，他们的功能进化的三个颚口OTx类的表达模式。表达模式进行了检查，在发展中的眼软骨鱼，角鲨，和一个小的，鸡，并与能力的旁系同源蛋白诱导色素表型在培养的视网膜细胞中的合作与bHLH-亮氨酸拉链蛋白Mitf。这种分析表明，每个OTx类的特点是高度特异性和保守的表达功能，在假定的RPE，其中OTx 1和OTx 2，但不是OTx 5，转录在视泡阶段，在分化的神经视网膜，其中OTx 2和OTx 5显示一个保守的动态表达模式，并在形成睫状突，一个主要网站的OTx 1表达。此外，角鲨和小鼠的旁系同源蛋白在诱导色素表型的能力方面没有显示出任何显著差异，这表明RPE的规格和分化的功能等同。这些数据表明，特定的功能，选择性地涉及每个OTx的直系类固定前的gnathostorne辐射和突出的多基因家族的功能多样化的监管变化的突出作用。(C)2004爱思唯尔公司All rights reserved.
We have performed a detailed analysis of the expression pattern of the three gnathostome Otx classes in order to gain new insights into their functional evolution. Expression patterns were examined in the developing eye of a chondrichthyan, the dogfish, and an amniote, the chick, and compared with the capacity of paralogous proteins to induce a pigmented phenotype in cultured retina cells in cooperation with the bHLH-leucine zipper protein Mitf. This analysis indicates that each Otx class is characterized by highly specific and conserved expression features in the presumptive RPE, where Otx1 and Otx2, but not Otx5, are transcribed at optic vesicle stages, in the differentiating neural retina, where Otx2 and Otx5 show a conserved dynamic expression pattern, and in the forming ciliary process, a major site of Otx1 expression. Furthermore, the paralogous proteins of the dogfish and the mouse do not display any significant difference in their capacity to induce a pigmented phenotype, suggesting a functional equivalency in the specification and differentiation of the RPE. These data indicate that specific functions selectively involving each Otx orthology class were fixed prior to the gnathostorne radiation and highlight the prominent role of regulatory changes in the functional diversification of the multigene family. (C) 2004 Elsevier Inc. All rights reserved.