DREADDs for Neuroscientists.
DREADDs for Neuroscientists.
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DOI：
10.1016/j.neuron.2016.01.040
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发表时间：
2016-02-17
期刊：
影响因子：
16.2
通讯作者：
Roth BL
中科院分区：
文献类型：
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作者：
Roth BL
为了了解大脑功能，我们必须发现细胞信号如何指定正常和病理性的大脑功能。在这方面，化学遗传学技术代表了在自由移动的动物中以细胞类型特异性方式操纵神经元和非神经元信号转导的有价值的平台。基于化学遗传学工具的设计师受体专门由设计师药物激活（DREADD）现在通常被神经科学家用于识别从苍蝇到非人类灵长类动物的物种中指定行为，感知，情感，先天驱动和运动功能的电路和细胞信号。在这里，我提供了一个关于DREADDs的初级读本，强调了关键的技术和概念考虑，并确定了化学遗传学未来的挑战。
To understand brain function, it is essential that we discover how cellular signaling specifies normal and pathological brain function. In this regard, chemogenetic technologies represent valuable platforms for manipulating neuronal and non-neuronal signal transduction in a cell-type-specific fashion in freely moving animals. Designer Receptors Exclusively Activated by Designer Drugs (DREADD)-based chemogenetic tools are now commonly used by neuroscientists to identify the circuitry and cellular signals that specify behavior, perceptions, emotions, innate drives, and motor functions in species ranging from flies to nonhuman primates. Here I provide a primer on DREADDs highlighting key technical and conceptual considerations and identify challenges for chemogenetics going forward.