High-density single-unit human cortical recordings using the Neuropixels probe
High-density single-unit human cortical recordings using the Neuropixels probe
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DOI：
10.1016/j.neuron.2022.05.007
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发表时间：
2022-08-03
期刊：
影响因子：
16.2
通讯作者：
Chang, Edward F.
中科院分区：
文献类型：
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作者：
Chung, Jason E.;Sellers, Kristin K.;Chang, Edward F.
动作电位是神经计算的基本单位。尽管在动物模型中记录大量单个神经元方面已经取得了重大进展，但由于临床限制和电极可靠性，这些方法向人类的转化受到限制。在这里，我们提出了一种可靠的方法，使用Neuropixels探针在术中记录数十个人类神经元，产生高达100个同时记录的单个单位。大多数单个装置在到达目标深度的1分钟内活动。电极阵列的运动与产率具有很强的负相关性，从而确定了进一步提高探针实用性的主要挑战和机会。在大多数记录中，在时间上接近的活跃细胞对在空间上更接近，这表明了解决复杂皮层动力学的能力。总而言之，这种方法提供了在人类新皮层的深度上以以前只能在动物模型中获得的尺度获得群体单单位活动的途径。
The action potential is a fundamental unit of neural computation. Even though significant advances have been made in recording large numbers of individual neurons in animal models, translation of these methodologies to humans has been limited because of clinical constraints and electrode reliability. Here, we present a reliable method for intraoperative recording of dozens of neurons in humans using the Neuropixels probe, yielding up to similar to 100 simultaneously recorded single units. Most single units were active within 1 min of reaching target depth. The motion of the electrode array had a strong inverse correlation with yield, identifying a major challenge and opportunity to further increase the probe utility. Cell pairs active close in time were spatially closer in most recordings, demonstrating the power to resolve complex cortical dynamics. Altogether, this approach provides access to population single-unit activity across the depth of human neocortex at scales previously only accessible in animal models.