Glutathione permeability of CFTR
Glutathione permeability of CFTR
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DOI：
10.1152/ajpcell.1998.275.1.c323
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发表时间：
1998-07-01
影响因子：
5.5
通讯作者：
Hanrahan, JW
中科院分区：
文献类型：
--
作者：
Linsdell, P;Hanrahan, JW
囊性纤维化跨膜传导调节因子（CFTR）形成一个离子通道，可渗透Cl（-）和较大的有机阴离子。在这里，我们表明，使用宏观电流记录从切除的膜补丁，阴离子抗氧化剂三肽谷胱甘肽是渗透在CFTR通道。这种渗透性可能是在气道上皮细胞表面液体中测量到的高浓度谷胱甘肽的原因。此外，谷胱甘肽转运途径的丧失可能导致囊性纤维化患者气道表面液中谷胱甘肽水平降低，这可能导致囊性纤维化患者肺中观察到的氧化应激。我们认为，谷胱甘肽释放到气道表面液体可能是CFTR的一个新功能。
The cystic fibrosis transmembrane conductance regulator (CFTR) forms an ion channel that is permeable both to Cl(-) and to larger organic anions. Here we show, using macroscopic current recording from excised membrane patches, that the anionic antioxidant tripeptide glutathione is permeant in the CFTR channel. This permeability may account for the high concentrations of glutathione that have been measured in the surface fluid that coats airway epithelial cells. Furthermore, loss of this pathway for glutathione transport may contribute to the reduced levels of glutathione observed in airway surface fluid of cystic fibrosis patients, which has been suggested to contribute to the oxidative stress observed in the lung in cystic fibrosis. We suggest that release of glutathione into airway surface fluid may be a novel function of CFTR.