SPINAL PROJECTION NEURONS TO THE LATERODORSAL PONTINE TEGMENTAL NUCLEUS - RELATIONSHIP TO PREGANGLIONIC NEURONS AND NITRIC-OXIDE SYNTHASE
SPINAL PROJECTION NEURONS TO THE LATERODORSAL PONTINE TEGMENTAL NUCLEUS - RELATIONSHIP TO PREGANGLIONIC NEURONS AND NITRIC-OXIDE SYNTHASE
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DOI：
10.1002/cne.903530102
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发表时间：
1995-02-27
影响因子：
2.5
通讯作者：
MCNEILL, DL
中科院分区：
文献类型：
--
作者：
HAMILTON, MO;PAPKA, RE;MCNEILL, DL
大鼠骶副交感神经核（SPN）的区域包含不同的神经元亚群，这些神经元是脊髓上投射的或节前神经元。SPN中的一些节前神经元作为膀胱收缩的运动流出; SPN中的其他神经元投射到嘴侧笔的区域，从而促进排尿反射。以前的研究利用免疫组织化学或染色的烟酰胺腺嘌呤二核苷酸磷酸黄递酶（NADPH-d）已经证明，许多神经元在SPN含有一氧化氮合酶（NOS），一氧化氮合成酶。因此，本研究的目的是确定1）投射到脑桥背侧被盖（LDT）的SPN区域中神经元的分布，2）投射到外周自主神经节的脊髓神经元是否也投射到LDT，以及3）NOS或NADPH-d是否存在于LDT投射神经元中。通过将逆行示踪剂荧光金（FG）注入盆大神经节（MPG）来鉴定节前神经元。通过将逆行示踪剂快蓝（FB）注射到LDT中来鉴定棘上投射神经元。许多FB-标记的神经元存在于同侧和对侧的SPN，并立即背对FG-标记的节前神经元。未观察到含有两种示踪剂的神经元。大约20%的节前神经元，但没有LDT投射神经元，反应NOS和NADPH-d。这些数据表明，该地区的SPN是一个网站的不同亚群的神经元，项目的LDT和MPG和NOS是包含在一些节前神经元，但不是一个标记LDT投射神经元。(C)1995 Wiley-Liss，Inc.
The region of the rat sacral parasympathetic nucleus (SPN) contains distinct subpopulations of neurons that project supraspinally or are preganglionic neurons. Some preganglionic neurons in the SPN serve as the motor outflow for urinary bladder contraction; other neurons in the SPN project to regions of the rostral pens that subserve micturition reflexes. Previous studies utilizing immunohistochemistry or staining for nicotinamide adenine dinucleotide phosphate-diaphorase (NADPH-d) have demonstrated that numerous neurons in the SPN contain nitric oxide synthase (NOS), the enzyme for nitric oxide synthesis. Thus, the objectives of this study were to determine 1) the distribution of neurons in the region of the SPN that project to the laterodorsal tegmentum (LDT) of the pons, 2) whether spinal neurons projecting to a peripheral autonomic ganglion also project to the LDT, and 3) whether NOS or NADPH-d is present in LDT projection neurons. Preganglionic neurons were identified by injecting the retrograde tracer fluorogold (FG) into the major pelvic ganglion (MPG). Supraspinally projecting neurons were identified by injecting the retrograde tracer fast blue (FB) into the LDT. Numerous FB-labeled neurons were present in the ipsi- and contralateral SPN and were immediately dorsal to FG-labeled preganglionic neurons. Neurons containing both tracers were not observed. Approximately 20% of preganglionic neurons, but no LDT projection neurons, were reactive for NOS and NADPH-d. These data suggest that the region of the SPN is a site for distinct subpopulations of neurons that project to the LDT and to the MPG and that NOS is contained in some preganglionic neurons, but is not a marker for LDT projection neurons. (C) 1995 Wiley-Liss, Inc.