Structural effects on ss- and dsDNA recognition by a beta-hairpin peptide.
Structural effects on ss- and dsDNA recognition by a beta-hairpin peptide.
复制标题
β-发夹肽对 ss- 和 dsDNA 识别的结构影响。
DOI：
10.1002/cbic.200800524
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发表时间：
2009
期刊：
影响因子：
--
通讯作者：
Waters,MarceyL
中科院分区：
文献类型：
--
作者：
Stewart,AmandaL;Waters,MarceyL
Form defines function: The effects of β‐hairpin structure on the binding affinity and selectivity for ssDNA versus dsDNA were investigated; this provided insights into the factors that contribute to the selective recognition of both ss‐ and dsDNA and suggested new approaches for designing biomimetic receptors. Binding studies showed that 1) folding is crucial for binding to both ss‐ and dsDNA, and 2) chirality affects binding for duplex but not for ssDNA.The interactions involved in the binding of a designed β‐hairpin dimer to single‐stranded and duplex DNA have been explored. Previously the peptide dimer had been found to bind ssDNA with a dissociation constant of 3 μμthrough a combination of aromatic and electrostatic interactions, whereas binding to duplex DNA was primarily driven by electrostatic interactions. In this report, the effects of folding and chirality were studied to determine the factors that contribute to affinity and selectivity for ssDNA versus dsDNA. Binding studies showed that 1) folding is crucial for binding to both ss‐ and dsDNA, and 2) chirality affects binding for duplex DNA but not for ssDNA. Taken together, these studies reveal different modes of binding for ss‐ and duplex DNA, with different driving forces, but in each case peptide structure contributes significantly to binding.
