Identification of a BALB/c-3T3 cell protein modulated by platelet-derived growth factor.
Identification of a BALB/c-3T3 cell protein modulated by platelet-derived growth factor.
复制标题
血小板衍生生长因子调节的 BALB/c-3T3 细胞蛋白的鉴定。
DOI：
10.1128/mcb.3.1.70-81.1983
复制
发表时间：
1983
影响因子：
5.3
通讯作者：
Locatell,KL
中科院分区：
文献类型：
--
作者：
Scher,CD;Dick,RL;Whipple,AP;Locatell,KL
血小板衍生生长因子（PDGF）刺激密度阻滞的BALB/c-3T3细胞合成一种蛋白质（pII;Mr, 35,000），这种蛋白质是由自发转化的BALB/c-3T3 （ST2-3T3）细胞合成的，而这些细胞的生长不需要PDGF。针对逆转录病毒转化细胞的主要排泄蛋白家族（MEP）的抗血清定量地沉淀细胞pII。pdgf刺激的pII与从ST2-3T3或逆转录病毒转化细胞中分离的MEP具有相同的分子量、相似的电荷和相似的抗原决定因子。MEP约占ST2-3T3细胞合成的非核蛋白的2%，占pdgf处理的BALB/c-3T3细胞合成的蛋白的0.3 - 0.6%，比背景增加了3 - 6倍。在BALB/c-3T3细胞中，pII （MEP）合成所需的PDGF少于DNA合成所需的PDGF。PDGF在40分钟内诱导pII （MEP）选择性增加。这种优先合成被放线菌素D短暂处理抑制，表明需要新形成的RNA。放线菌素d不抑制ST2-3T3细胞组成性合成pII （MEP）。对5株自发或化学致癌转化的致瘤性BALB/c-3T3细胞株进行了研究；它们既不需要PDGF来生长，也对它没有反应。这些细胞系在与G1DNA汇合时被抑制。这些独立分离的线组成合成pII （MEP）。因此，对于pdgf调节的DNA合成，pII （MEP）的合成可能是必需的，但不是充分的。
The platelet-derived growth factor (PDGF) stimulates density-arrested BALB/c-3T3 cells to synthesize a protein (pII;Mr, 35,000) that is constitutively synthesized by spontaneously transformed BALB/c-3T3 (ST2-3T3) cells which do not require PDGF for growth. Antisera against a major excreted protein family (MEP) of retrovirus-transformed cells quantitatively precipitated cellular pII. PDGF-stimulated pII has the same molecular weight, a similar charge, and similar antigenic determinants as authentic MEP isolated from ST2-3T3 or retrovirus-transformed cells. MEP represented about 2% of the nonnuclear proteins synthesized by ST2-3T3 cells and 0.3 to 0.6% of the proteins synthesized by PDGF-treated BALB/c-3T3 cells, a three- to sixfold increase over the background. In BALB/c-3T3 cells, less PDGF was required for pII (MEP) synthesis than for DNA synthesis. PDGF induced a selective increase in pII (MEP) within 40 min. Such preferential synthesis was inhibited by brief treatment with actinomycin D, suggesting a requirement for newly formed RNA. The constitutive synthesis of pII (MEP) by ST2-3T3 cells was not inhibited by actinomycin D. Five spontaneously or chemical carcinogen-transformed tumorigenic BALB/c-3T3 cell lines were studied; they neither required PDGF for growth nor responded to it. These cell lines became arrested at confluence with a G1DNA content. Each of these independently isolated lines synthesized pII (MEP) constitutively. Thus, the synthesis of pII (MEP) may be required, but is not sufficient, for PDGF-modulated DNA synthesis.