Transcriptome analysis of differentially expressed circRNAs miRNAs and mRNAs during the challenge of coccidiosis.
Transcriptome analysis of differentially expressed circRNAs miRNAs and mRNAs during the challenge of coccidiosis.
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DOI：
10.3389/fimmu.2022.910860
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发表时间：
2022
影响因子：
7.3
通讯作者：
中科院分区：
文献类型：
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禽球虫病是由艾美耳球虫属寄生虫感染引起的一种常见的地方性疾病。它给全球家禽业造成了巨大的经济损失。目前使用抗球虫药物或接种疫苗的控制由于耐药性和疫苗相对昂贵而受到限制。提高宿主对艾美耳球虫的遗传抗性被认为是改善球虫病控制的有效策略。环状rna （circRNAs）已被发现作为多种疾病的生物标志物或诊断功能。在艾美耳球虫攻种过程中，与萨索鸡相关的环状rna、mirna和mrna的分子生物学功能尚未被描述。在这项研究中，RNA-seq分析了感染和未感染的商用Sasso T445艾美耳球虫种鸡脾脏中环状rna、mirna和mrna的表达模式。结果显示，感染和未感染的鸡共存在40个差异表达环状rna （decircrna）、31个差异表达mirna （demirna）和820个差异表达基因（demrna）。在差异表达的环状rna、mirna和mrna之间构建了调控网络，以深入了解鸡与艾美耳球虫之间的相互作用机制。对显著差异表达的环状rna circMGAT5的功能验证表明，环状rna circMGAT5可以海绵化miR-132c-5p，促进miR-132c-5p靶基因单核细胞向巨噬细胞分化相关（MMD）的表达。病理上，敲低circMGAT5可显著上调巨噬细胞表面标记物及巨噬细胞活化标记物F4/80和MHC-II的表达，提示circMGAT5可能抑制巨噬细胞的活化。miR-132c-5p显著促进F4/80和MHC-II的表达，而circMGAT5可以减弱miR-132c-5p诱导的F4/80和MHC-II的表达，表明circMGAT5通过circMGAT5-miR-132c-5p- mmd轴发挥功能。总之，我们的研究结果表明，环状rna在E. tenella感染过程中表现出抗性或易感作用。其中，circMGAT5可能通过circMGAT5- mir -132c-5p- mmd轴抑制巨噬细胞的活化，参与艾美球虫感染诱导的免疫应答。
Avian coccidiosis is a common enzootic disease caused by infection of Eimeria species parasites. It causes huge economic losses in the global poultry industry. Current control using anticoccidial drugs or vaccination is limited due to drug resistance and the relatively high cost of vaccines. Improving host genetic resistance to Eimeria species is considered an effective strategy for improved control of coccidiosis. Circular RNAs (circRNAs) have been found to function as biomarkers or diagnoses of various kinds of diseases. The molecular biological functions of circRNAs, miRNAs, and mRNAs related to Sasso chicken have not yet been described during Eimeria species challenge. In this study, RNA-seq was used to profile the expression pattern of circRNAs, miRNAs, and mRNAs in spleens from Eimeria tenella-infected and non-infected commercial dual-purpose Sasso T445 breed chickens. Results showed a total of 40 differentially expressed circRNAs (DEcircRNAs), 31 differentially expressed miRNAs (DEmiRNAs), and 820 differentially expressed genes (DEmRNAs) between infected and non-infected chickens. Regulatory networks were constructed between differentially expressed circRNAs, miRNAs, and mRNAs to offer insights into the interaction mechanisms between chickens and Eimeria spp. Functional validation of a significantly differentially expressed circRNA, circMGAT5, revealed that circMGAT5 could sponge miR-132c-5p to promote the expression of the miR-132c-5p target gene monocyte to macrophage differentiation-associated (MMD) during the infection of E. tenella sporozoites or LPS stimulation. Pathologically, knockdown of circMGAT5 significantly upregulated the expression of macrophage surface markers and the macrophage activation marker, F4/80 and MHC-II, which indicated that circMGAT5 might inhibit the activation of macrophage. miR-132c-5p markedly facilitated the expression of F4/80 and MHC-II while circMGAT5 could attenuate the increase of F4/80 and MHC-II induced by miR-132c-5p, indicating that circMGAT5 exhibited function through the circMGAT5-miR-132c-5p-MMD axis. Together, our results indicate that circRNAs exhibit their resistance or susceptive roles during E. tenella infection. Among these, circMGAT5 may inhibit the activation of macrophages through the circMGAT5-miR-132c-5p-MMD axis to participate in the immune response induced by Eimeria infection.
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DOI：
10.1186/s12711-018-0433-7
发表时间：
2018-11-21
期刊：
Genetics, selection, evolution : GSE
影响因子：
--
作者：
Boulton K;Nolan MJ;Wu Z;Psifidi A;Riggio V;Harman K;Bishop SC;Kaiser P;Abrahamsen MS;Hawken R;Watson KA;Tomley FM;Blake DP;Hume DA
通讯作者：
Hume DA
全面鉴定环状RNA的内部结构和选择性剪接事件
影响因子：
16.6
作者：
Gao Y;Wang J;Zheng Y;Zhang J;Chen S;Zhao F
通讯作者：
Zhao F
影响因子：
3.2
作者：
Fan, Xian-Cheng;Liu, Ting-Li;Zhao, Guang-Hui
通讯作者：
Zhao, Guang-Hui
DOI：
10.1073/pnas.1506468112
发表时间：
2015-09-22
影响因子：
11.1
作者：
Blake, Damer P.;Clark, Emily L.;Tomley, Fiona M.
通讯作者：
Tomley, Fiona M.
影响因子：
--
作者：
Aunin E;Böhme U;Blake D;Dove A;Smith M;Corton C;Oliver K;Betteridge E;Quail MA;McCarthy SA;Wood J;Tracey A;Torrance J;Sims Y;Howe K;Challis R;Berriman M;Reid A
通讯作者：
Reid A