Identification of the miRNA-3185/CYP4A11 axis in cardiac tissue as a biomarker for mechanical asphyxia
Identification of the miRNA-3185/CYP4A11 axis in cardiac tissue as a biomarker for mechanical asphyxia
复制标题
心脏组织中 miRNA-3185/CYP4A11 轴的鉴定作为机械性窒息的生物标志物
DOI：
10.1016/j.forsciint.2020.110293
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发表时间：
2020-06-01
影响因子：
2.2
通讯作者：
Chen, Long
中科院分区：
文献类型：
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作者：
Han, Liujun;Zhang, Heng;Chen, Long
机械性窒息死亡是法医科学中最难得出的结论之一，特别是在身体表面有轻微创伤或没有创伤的尸体上。因此，机械性窒息死亡在法医学实践中难以证明。MicroRNAs （miRNAs）是一类小的非编码rna，参与调控许多生理和病理细胞过程。在本研究中，我们证明，与颅脑损伤、失血性休克、心源性猝死和中毒病例相比，机械性窒息病例中心脏组织中miR-3185的表达显著增加。我们观察到miR-3185的表达与死后时间、年龄或温度之间没有相关性。进一步研究表明CYP4A11可能是miR-3185的靶基因，与颅脑损伤、失血性休克、心源性猝死和中毒病例的心脏组织标本相比，CYP4A11在机械性窒息病例的心脏组织标本中的表达水平相对较低。我们的研究结果表明，miRNA-3185/CYP4A11轴与机械性窒息性死亡有关，并可能为窒息性死亡研究提供新的见解。(C) 2020 Elsevier B.V.版权所有
Death by mechanical asphyxia is one of the most difficult conclusions to make in forensic science, especially in corpses displaying slight or no trauma to the surface of the body. Therefore, death by mechanical asphyxia is difficult to prove in medico-legal practice. MicroRNAs (miRNAs) are a class of small, non-coding RNAs involved in the regulation of numerous physiological and pathological cellular processes. In the present study, we demonstrate that significantly increased expression of miR-3185 in cardiac tissues was detected among cases of mechanical asphyxia compared to case of craniocerebral injury, hemorrhagic shock, sudden cardiac death and poisoning. We observed no correlation between the expression of miR-3185 and postmortem interval, age or temperature. Further work indicated that CYP4A11 is a putative target gene of miR-3185 and expressed at a relatively low level in cardiac tissue specimens from cases of mechanical asphyxia compared with specimens from cases of craniocerebral injury, hemorrhagic shock, sudden cardiac death and poisoning. Our results suggest that the miRNA-3185/CYP4A11 axis is associated with mechanical asphyxia-induced death and may provide new insight into asphyxial death investigations. (C) 2020 Elsevier B.V. All rights reserved.