Pancreatic islet blood flow in conscious rats during hyperglycemia and hypoglycemia.
Pancreatic islet blood flow in conscious rats during hyperglycemia and hypoglycemia.
复制标题
高血糖和低血糖期间清醒大鼠的胰岛血流量。
DOI：
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发表时间：
2001
期刊：
影响因子：
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通讯作者：
M. Yoshinari
中科院分区：
文献类型：
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作者：
M. Iwase;K. Tashiro;Y. Uchizono;D. Goto;M. Yoshinari
麻醉影响全身血流动力学和器官灌注的调节。我们使用彩色微球测量清醒大鼠在高血糖或低血糖两个时间点的胰岛血流量。这种方法，使用黑色和绿色微球，通过与以前的微球实验比较和缺乏非代谢葡萄糖类似物，3-O-甲基葡萄糖，胰岛灌注的影响进行了验证。基础和葡萄糖刺激的胰岛血流量水平在戊巴比妥钠麻醉和清醒大鼠中相似。然而，麻醉改变了胰腺血流的基础分布（清醒大鼠胰岛血流分数为5.8 ± 0.4%，戊巴比妥麻醉大鼠为7.9 ± 0.8%，P <0.05）。胰岛素诱导的低血糖显著增加了清醒大鼠的全胰腺血流量，而胰岛血流量保持不变，部分胰岛血流量减少（基础状态为5.8 ± 0.5%，低血糖期间为4.2 ± 0.4%，P <0.001）。甲基阿托品预处理显著增加低血糖时胰岛血流量181%。这一结果表明，预防低血糖诱导的胰岛灌注增加可能是介导的，至少部分是由胆碱能，迷走毒蕈碱机制。
Anesthesia affects general hemodynamics and regulation of organ perfusion. We used colored microspheres to measure pancreatic islet blood flow in conscious rats at two time points, during either hyperglycemia or hypoglycemia. This method, using black and green microspheres, was validated by comparison with previous microsphere experiments and by lack of effect of a nonmetabolizable glucose analog, 3-O-methylglucose, on islet perfusion. Basal and glucose-stimulated islet blood flow levels were similar in pentobarbital sodium-anesthetized and conscious rats. However, the basal distribution of pancreatic blood flow was altered by anesthesia (fractional islet blood flow 5.8 +/- 0.4% in conscious rats, 7.9 +/- 0.8% in pentobarbital-anesthetized rats, P < 0.05). Insulin-induced hypoglycemia significantly increased whole pancreatic blood flow in conscious rats, whereas islet blood flow remained unchanged and fractional islet blood flow was decreased (5.8 +/- 0.5% in the basal state, 4.2 +/- 0.4% during hypoglycemia, P < 0.001). Methylatropine pretreatment significantly increased islet blood flow during hypoglycemia by 181%. This result suggests that prevention of hypoglycemia-induced increase in islet perfusion may be mediated, at least in part, by a cholinergic, vagal muscarinic mechanism.
葡萄糖引起的胰岛充血是由一氧化氮介导的。
影响因子：
3
作者：
Moldovan,S;Livingston,E;Zhang,RS;Kleinman,R;Guth,P;Brunicardi,FC
通讯作者：
Brunicardi,FC