Measurement of receptor-activated phosphoinositide turnover in rat brain: nonequivalence of inositol phosphate and CDP-diacylglycerol formation.
Measurement of receptor-activated phosphoinositide turnover in rat brain: nonequivalence of inositol phosphate and CDP-diacylglycerol formation.
复制标题
大鼠脑中受体激活的磷酸肌醇周转的测量：磷酸肌醇和 CDP-二酰基甘油形成的不等价性。
DOI：
10.1111/j.1471-4159.1993.tb03258.x
复制
发表时间：
1993
影响因子：
4.7
通讯作者：
Agranoff,BW
中科院分区：
文献类型：
--
作者：
Heacock,AM;Seguin,EB;Agranoff,BW
Two methods for the measurement of receptor‐activated phosphoinositide turnover were evaluated for their degree of correspondence in slices of rat brain; they involved the Li+‐dependent accumulations of either [3H]‐inositol‐labeled inositol phosphates or [3H]cytidine‐labeled CDP‐diacylglycerol. In contrast to the expectation that the ratio of these two responses would remain approximately constant, varying degrees of correspondence were obtained. The two extremes are exemplified by carbachol, which elicited large increases in both inositol phosphate and CDP‐diacylglycerol labeling, and endothelin, which gave a robust inositol phosphate response with little or no accumulation of3H‐CDP‐diacylglycerol. No instance of the presence of the latter response in the absence of3H‐inositol phosphate accumulation was observed. Measurement of3H‐CDP‐diacylglycerol accumulation thus may add additional insight into the regulation of phosphoinositide turnover and the complex actions of Li+.
