FUNCTIONAL DEVELOPMENT IN MAUTHNER CELL SYSTEM OF EMBRYOS AND LARVAE OF ZEBRA FISH
FUNCTIONAL DEVELOPMENT IN MAUTHNER CELL SYSTEM OF EMBRYOS AND LARVAE OF ZEBRA FISH
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DOI：
10.1002/neu.480080207
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发表时间：
1977-01-01
期刊：
影响因子：
--
通讯作者：
SCHABTACH, E
中科院分区：
文献类型：
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作者：
EATON, RC;FARLEY, RD;SCHABTACH, E
在斑马鱼胚胎[Brachydanio rerio]中，早在受精后40小时，毛特纳细胞（m细胞）就在触觉振动刺激下启动了逃逸反应。这种行为的最初部分类似于幼虫阶段的声音惊吓反射，开始于96小时。胚胎反应是定向的，随后是一系列强烈的尾巴弯曲，比游泳时更明显。在胚胎中，m细胞在反应开始时发射，在随后的收缩中很少再发射；在这些实验中，m细胞并没有调节尾巴的反复运动。m细胞系统可能与孵化行为有关，因为触觉反应足以使卵膜破裂并使动物逃脱。m细胞有时自发放电，这表明它也可能在没有相刺激时发生的自发孵化行为中起作用。48h时，m细胞在胞体和树突上有形态成熟的突触，但胞质未分化；定向神经丝少，无明显的轴突丘。在这两个阶段，细胞外m峰的持续时间更长，幅度较小，而在细胞形态成熟的后期。m细胞系统的长期抑制控制显然在大约孵化时开始起作用。此时细胞在形态上已经成熟，在其体细胞和树突上有丰富的突触末梢。
In the embryonic zebra fish [Brachydanio rerio] as early as 40 h after fertilization, the Mauthner cells (M-cells) initiated an escape response, elicited by tactile-vibrational stimulation. The initial part of this behavior was similar to the acoustic startle reflex seen during the larval stage which begins at 96 h. The embryonic response was directional and was followed by a series of strong tail flexures which were more pronounced than those during swimming. In the embryo the M-cell fired at the beginning of the response and rarely fired again during subsequent contractions; in these experiments the M-cell did not mediate iterative movements of the tail. The M-cell system is probably involved in evoked hatching behavior, as the tactile response was sufficient to rupture the egg membrane and allow the animal to escape. The M-cell sometimes fired spontaneously, which suggested that it might function also in spontaneous hatching behavior which occurs in the absence of phasic stimulation. At 48 h the M-cell had morphologically mature synapses on its soma and dendrites, but its cytoplasm was relatively undifferentiated; it had few oriented neurofilaments and no distinct axon hillock. During these stages the extracellular M-spike was longer in duration and smaller in amplitude than at later times when the cell was more mature morphologically. Long-term inhibitory control of the M-cell system apparently begins to function at about the time of hatching. At this time the cell was morphologically mature and was richly supplied with synaptic endings over its soma and dendrites.