Transcriptional regulation and function during the human cell cycle
Transcriptional regulation and function during the human cell cycle
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DOI：
10.1038/83751
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发表时间：
2001-01-01
期刊：
影响因子：
30.8
通讯作者：
Lockhart, DJ
中科院分区：
文献类型：
--
作者：
Cho, RJ;Huang, MX;Lockhart, DJ
我们在这里报告的转录分析的细胞周期在全基因组范围内的人类成纤维细胞。我们确定了大约700个基因，显示转录波动与细胞周期的周期一致。这些基因的系统分析揭示了共调节转录组内的功能组织。一组不同的细胞骨架重组基因表现出细胞周期依赖性调节，表明生物学途径被重定向用于执行细胞分裂。参与细胞运动和细胞外基质重塑的许多基因主要在M期表达，表明平衡增殖和侵袭细胞行为的机制。在S期上调的成绩单显示广泛的重叠与DNA损伤诱导的基因，细胞周期调控的成绩单，因此可能构成连贯的程序，用于响应外部刺激。我们的数据还为数百个以前未被表征的人类基因的生物功能提供了线索。
We report here the transcriptional profiling of the cell cycle on a genome-wide scale in human fibroblasts. We identified approximately 700 genes that display transcriptional fluctuation with a periodicity consistent with that of the cell cycle. Systematic analysis of these genes revealed functional organization within groups of coregulated transcripts. A diverse set of cytoskeletal reorganization genes exhibit cell-cycle-dependent regulation, indicating that biological pathways are redirected for the execution of cell division. Many genes involved in cell motility and remodeling of the extracellular matrix are expressed predominantly in M phase, indicating a mechanism for balancing proliferative and invasive cellular behavior. Transcripts upregulated during S phase displayed extensive overlap with genes induced by DNA damage; cell-cycle-regulated transcripts may therefore constitute coherent programs used in response to external stimuli. Our data also provide clues to biological function for hundreds of previously uncharacterized human genes.