Interactions between 17 beta-estradiol and progesterone in the control of luteinizing hormone and follicle-stimulating hormone release in rat anterior pituitary cells in culture.
Interactions between 17 beta-estradiol and progesterone in the control of luteinizing hormone and follicle-stimulating hormone release in rat anterior pituitary cells in culture.
复制标题
17β-雌二醇和黄体酮之间的相互作用在控制培养的大鼠垂体前叶细胞中黄体生成素和卵泡刺激素释放中的相互作用。
作者：
Jacques Drouin;F. Labrie
大鼠垂体前叶细胞经10(-9)M - 17 β -雌二醇（E2）预孵育48小时后，基础LH释放增加1- 2倍，而诱导LH释放半最大刺激（ED50）所需的LHRH浓度约降低50%。单独存在10(-7)M孕酮(P) 48 h对LH释放无影响，但抑制E2对LH对LHRH的增敏作用。P对e2诱导的LH对LHRH反应性增加的抑制作用与雄激素的作用相似。虽然不影响LHRH促卵泡刺激素释放的ED50，但P刺激基础卵泡刺激素释放和对LHRH的最大卵泡刺激素反应。P的这种作用在E2的同时孵育下增强。单独E2对LH释放的刺激作用ED50值为1-2 × 10(-11) m， E2对p诱导的促卵泡刺激素释放的增强作用ED50值相似。E2对LH对LHRH的反应性的刺激作用似乎是由于促性腺激素释放机制敏感性的改变，因为在相同的实验条件下，总激素含量（释放加上细胞含量）不受E2的影响。P对促卵泡刺激素释放的刺激作用在ED50值为1 × 10(-8) M时测量，并在与类固醇共孵育约8小时后达到最大。虽然不影响总LH，但P增加了总FSH（释放量加上细胞含量），表明P对FSH释放的刺激作用可能是继发于细胞激素含量的变化。
After preincubation of rat anterior pituitary cells in culture for 48 h with 10(-9) M 17 beta-estradiol (E2), basal LH release is increased 1- to 2-fold, while the concentration of LHRH required to induce a half-maximal stimulation (ED50) of LH release is approximately 50% reduced. The presence of 10(-7) M progesterone (P) alone for 48 h has no effect on LH release, but it inhibits the sensitizing action of E2 on the LH response to LHRH. This inhibitory effect of P on the E2-induced increase of LH responsiveness to LHRH is similar to the effect of androgens. While not affecting the LHRH ED50 for FSH release, P stimulates both basal FSH release and the maximal FSH response to LHRH. This effect of P is potentiated by simultaneous incubation in the presence of E2. The stimulatory effect of E2 alone on LH release is exerted at an ED50 value of 1-2 x 10(-11) M. A similar ED50 value is found for the potentiating effect of E2 on the P-induced stimulation of FSH release. The stimulatory effect of E2 on LH responsiveness to LHRH appears to be due to changes in the sensitivity of the release mechanisms in gonadotrophs, since the total hormone content (release plus cell content) is not affected by E2 under the same experimental conditions. The stimulatory effect of P on FSH release is measured at an ED50 value of 1 x 10(-8) M and is maximal after approximately 8 h of incubation with the steroid. While not affecting total LH, P increases total FSH (release plus cell content), indicating that the stimulatory effect of P on FSH release could be secondary to changes in the hormone content of cells.